30L生物反应器连续灌注培养重组CHO-C_(28)细胞表达HBsAg的研究  

HBsAg expression by CHO C 28 continuous cell culture applying 30 L bioreactor

作　　者：官桂范[1] 李志强[1] 王妍[1] 李云富[1] 耿星涛 陈万革[1] 叶世德[1] 

机构地区：[1]长春生物制品研究所,长春130062

出　　处：《微生物学免疫学进展》1999年第4期45-54,共10页Progress In Microbiology and Immunology

摘　　要：应用３０ Ｌ生物反应器和微载体悬浮 培养技术，通过电脑全自动控制，连续灌注培养分泌ＨＢｓＡｇ 的重组ＣＨＯ－Ｃ２８细胞。试验了培养方式、连续灌流速度，反应器转速和细胞对葡萄糖消耗等工艺条件。观察培养６０ 天，细胞的生长形态、ＨＢｓＡｇ 分泌动态和染色体数。研究结果表明，连续培养６０ 天，细胞密度可达７．０×１０６ｃｅｌｌｓ／ｍ ｌ，平均维持在（５．０～６．０）×１０６ｃｅｌｌｓ／ｍ ｌ，收液的ＲＰＨＡ 滴度可达１∶５１２，ＨＢｓＡｇ 每天平均产量为３０ ｍ ｇ。The techniques and procedure culturing recombinant CHO C 28 cell or suspending microcarriers in 30 L bioreactor to secrete HBsAg has been studied,bioreact was automatic controled by computer.The culture mode ,the perfusion rate,the stirring speed and consumption of glucose etc.,have been investigated.Meanwhile the growth state of cell,the secretion and expression of HBsAg and the numbers of chromosome were observed during CHO cell continuous culturing 60 days period.The results proved that the cell density was 7.0×10 6 cells/ml,average maintaining was 5.0～6.0×10 6cells /ml. RPHA titre of HBsAg in harvested liquid was 1∶512,average output of HBsAg protein was 30 mg/day.

关 键 词：CHO-C28细胞 表面抗原 乙型肝炎 生物反应器 

分 类 号：TQ464[化学工程—制药化工] R373.21[医药卫生—病原生物学]