黄芩中黄芩苷的正交提取工艺及体外抑菌活性研究  

Optimization of the extracting process for Baicalin by orthogonal test and study on its antibacterial activity in vitro

作　　者：杨明[1] 陶蕾[2,3] 王文[4] 赵强[3] 陈虹[3] 

机构地区：[1]甘肃省畜牧兽医研究所,甘肃平凉744000 [2]中国农业科学院兰州畜牧与兽药研究所 [3]甘肃农业大学动物医学院 [4]甘肃农业大学草业学院

出　　处：《中兽医医药杂志》2011年第3期43-45,共3页Journal of Traditional Chinese Veterinary Medicine

基　　金：甘肃省技术研究与开发专项计划项目(QS031-C31-11)

摘　　要：采用正交提取法提取黄芩中黄芩苷,HPLC法检测其含量,并测定提取物对大肠埃希氏杆菌、金黄色葡萄球菌两种供试菌的体外最小抑菌浓度(MIC)。结果表明,最佳正交提取工艺为A2B2C3,即乙醇浓度70%、体积100 mL、冷浸36 h,提取物对大肠埃希氏杆菌、金黄色葡萄球菌的MIC分别为0.50 mg/mL和0.20 mg/mL。The extracting process was optimized for baicalin from Scutellaria baicalensi by orthogonal test. The contents of baicalin were determined by HPLC and its minimal inhibitory concentration （MIC） was measured by means of bacteriostasis tests in vitro with two kinds of bacteria. The results showed that the optimum extracting conditions were A2B2C3, that was the cold-maceration, 70% alcohol 100 mL as the solvents and to extract for 36 h; MIC of baicalin against Escherichia coli and Staphylococcus aureus was 0.50 and 0.20 mg/mL, respectively.

关 键 词：黄芩苷 正交提取 体外抑菌 

分 类 号：S853.74[农业科学—临床兽医学]