聚丙烯酸与核酸相互作用的共振光散射光谱研究及其分析应用  

Study on the Interaction between Polyacrylic Acid and Nucleic Acid by Resonance Light Scattering Spectra and Its Analytical Application

作　　者：翟云云[1] 王伟娟[2,1] 曾延波[1] 李蕾[1,2] 路齐英[2,1] 肖莹[2,1] 

机构地区：[1]嘉兴学院生物与化学工程学院,浙江嘉兴314001 [2]常州大学化学化工学院,江苏常州213016

出　　处：《分析科学学报》2011年第3期359-362,共4页Journal of Analytical Science

基　　金：浙江省科技计划重点项目(No.2007C23071);浙江省自然科学基金(No.Y4080252)

摘　　要：研究了聚丙烯酸(PAA)与脱氧核糖核酸(DNA)相互作用的共振光散射(RLS)光谱。实验结果表明,在pH=2.0的B-R缓冲溶液中,PAA与DNA自身的共振光散射峰均较弱,但当二者发生静电作用形成复合物后,体系的共振光散射峰增强,散射增强程度则各不相同,其相对散射强度的顺序是fsDNA>ctDNA>yRNA。在一定范围内核酸浓度与散射强度呈正比,fsDNA、ctDNA、yDNA的检出限(3σ)分别为:3.51、3.26、2.73μg/L,回收率为93.8%～104.1%。本方法已成功用于合成样品中痕量DNA的测定。A method based on the resonance light scattering（RLS） of PAA-DNA complex in Britton-Robinson buffer solution of pH=2.0 was proposed for the determination of DNA.The influences of some experimental factors,including pH value,PAA concentration,the sequence of adding the reagents and the coexistent substances,on the enhancement of the RLS intensity were studied.The RLS signal of PAA or nucleic acid alone was very weak,but it was greatly enhanced when they interacted with each other to form a bound product.There was a little difference of RLS spectra of the bound products for a variety of DNA and the increments of RLS intensity were followed by this sequence,fsDNActDNAyDNA.Furthermore,the RLS intensity is proportional to the concentration of DNA in a certain range,and a simple and rapid method for the determination of DNA was therefore developed,the detection limits of fsDNA,ctDNA,yDNA were 3.51 μg/L,3.26 μg/L and 2.73 μg/L,respectively with the recovery range of 93.8%~104.1%.The developed method has been applied to the determination of DNA in synthetic samples.

关 键 词：共振光散射 聚丙烯酸 核酸 

分 类 号：O657.39[理学—分析化学]