玫瑰精B光度法测定苯唑西林的含量  

Determination of Oxacillin by Rhodamine B Spectrophotometry

作　　者：庞向东[1] 邓钦心[1] 吴言兵[1] 江虹[1] 

机构地区：[1]长江师范学院化学化工学院,重庆408100

出　　处：《分析科学学报》2011年第3期406-408,共3页Journal of Analytical Science

基　　金：重庆市教委科研项目(No.KJ071304);长江师范学院科研项目(No.2008xky39)

摘　　要：在pH=9.13的Tris-HCl缓冲体系中,玫瑰精B可与苯唑西林反应生成浅粉红色离子缔合物,产生两个明显的褪色峰。其最大褪色波长分别位于578nm和494nm,表观摩尔吸光系数(ε)分别为8.70×104 L/(mol.cm)和5.20×104 L/(mol.cm)。苯唑西林的浓度在0～0.6mg/L范围内与溶液的褪色程度呈良好的线性关系。该方法简便、快速、灵敏,可用于苯唑西林的测定。In a pH=9.13 Tris-HCl buffer medium,rhodamine B reacteds with oxacillin to form baby pink ion-association complex with two fading peaks,and the maximum fading wavelength was located at 578 nm and 494 nm,respectively.The apparent molar absorption coefficient（ε） were 8.70×104 and 5.20×104 L/（mol·cm）,respectively.The fading intensity was linearly proportional to the concentration of oxacillin in a range of 0~0.6 mg/L.The method is simple,rapid and sensitive,and it was applied to the determination of oxacillin with satisfactory results.

关 键 词：苯唑西林 玫瑰精B 分光光度法 

分 类 号：O657.32[理学—分析化学]