多重引物PCR和异源双链毛细管电泳技术在B细胞淋巴瘤诊断中的应用  

Application of multiplex PCR and heteroduplex technique in detection of B-cell lymphoma

作　　者：马杰[1] 魏冰[1] 夏庆欣[1] 

机构地区：[1]河南省肿瘤医院病理科,郑州450008

出　　处：《医药论坛杂志》2011年第7期3-6,共4页Journal of Medical Forum

基　　金：河南省科技攻关项目(200803121)

摘　　要：目的探讨多重引物PCR和异源双链毛细管电泳技术(heteroduplex analysis HA)在B细胞淋巴瘤诊断中的应用。方法从普通石蜡切片组织中提取基因组DNA用多重引物PCR扩增。利用双链异源技术进行毛细管电泳,并对其产物进行分析。结果 32例B细胞淋巴瘤中29例检测有克隆性重排,检出率90.65%。10例反应性增生病例均成多克隆性重排。结论在B细胞受体IgH和IgK基因克隆重排检测中,多重引物PCR及异源双链毛细管电泳技术具有样本来源便利,灵敏度高,特异性强,耗时短等特点,可作为B细胞淋巴瘤的诊断及鉴别诊断的辅助检测手段。Objective To explore the application of multiplex PCR and heteroduplex technique in the detection of B-cell receptor IgH/Igk rearrangement.Methods DNA from paraffin embedded tissues were extracted and amplified by multiplex primer PCR.To use double stranded heterologous for capillary electrophoresis,and analyzed by hereroduplex and Gene-Scan.Results Totally 29 cases of B-cell lymphoma were positive for PCR rearragement to use heteroduplex technique detecting.10 cases of reactive lymphoid hyperplasia were negative.These results were corresponding to the detection by Gene-Scan.Conclusions Multiplex-PCR and heteroduplex technique is a sensitive and convinient tool for PCR rearrangement analysis.It can be useful in diagnosis and differential diagnosis of B-cell lymphoma.

关 键 词：异源双链毛细管电泳 B细胞淋巴瘤 克隆性重排 PCR 

分 类 号：R34[医药卫生—基础医学]