实时荧光定量PCR检测miR169g在脱水与高盐胁迫下胡杨叶中的表达  

Expression determination of miR169g under dehydration and high salinity stress in populus euphratica leaves by real-time quantitative PCR

作　　者：覃玉蓉[1] 夏新莉[1] 尹伟伦[1] 

机构地区：[1]北京林业大学生物科学与技术学院,北京100083

出　　处：《现代仪器》2011年第3期28-30,共3页Modern Instruments

基　　金：国家自然科学基金项目(30730077);国家"十一五"科技支撑计划课题(2006BAD03A01);国家林业局"948"项目(2007-4-01)

摘　　要：microRNAs是一类具有重要功能的约由22个核苷酸组成的小分子非编码RNAs,一些miRNAs在植物非生物胁迫中发挥着重要的作用。本文采用stem-loopSYBR Green实时RT-PCR方法检测miR169g在脱水与高盐处理下胡杨叶中的表达情况,结果表明,miR169g脱水与高盐胁迫下胡杨叶中均表达上调。此方法灵敏简便,结果稳定可靠,适用于检测短序列miRNAs成熟体的表达。MicroRNAs （miRNAs） are a class of-22nt non-coding small RNAs with important function, which have emerged as important players in plant abiotic environmental stress responses, In this study, the stem-loop SYBR Green real-time RT-PCR was applied to determination of miR169g expression under dehydration and high salinity stress in populus euphratica leaves. These results showed miR169g were up-regulated in leaves of Reuphratica under dehydration and high salinity stress. This method is sensitive, simple, stable and reliable for detecting the expression of the mature miRNAs.

关 键 词：实时 RT-PCR MiR169g 胡杨 脱水 高盐 

分 类 号：Q945.78[生物学—植物学]