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作 者:刘美剑[1] 常亚青[1] 杨芸菲[1] 刘艳萍[1] 张婧[1] 张伟杰[1] 王轶南[1]
机构地区:[1]大连海洋大学,农业部海洋水产增养殖学重点开放实验室,辽宁大连116023
出 处:《中国农业科技导报》2011年第3期129-135,共7页Journal of Agricultural Science and Technology
基 金:国家863计划项目(2006AA10A408);辽宁省重大项目(2008203001);农业部948项目(2006-G55A)资助
摘 要:盐度是影响贝类生长的重要环境因子之一,将虾夷扇贝在盐度40,35和30的条件下培养7 d后,进行盐度渐变处理,分别由40降至35、35降至30、30降至25、30升至35,检测虾夷扇贝在不同处理时间的溶菌酶(LZM)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽(GSH)活力。结果显示各处理组中4项免疫酶指标的变化趋势基本一致,即盐度渐变后LZM活力相对对照组(0 h)呈现显著下降后逐渐上升最终趋于平缓且高于对照;SOD活力短期内下降显著,而后逐渐趋于对照水平;CAT活力先下降后恢复再下降,最终趋于对照水平;GSH活力短期内稳定后逐渐升高后恢复至对照水平。上述结果表明,盐度渐变对虾夷扇贝LZM、SOD、CAT及GSH等四项免疫指标有显著影响。Salinity is one of the important environmental factors interfering the growth of scallops.The scallops were temporarily cultured at different salinity levels 40,35,30 for 7 days,and the activities of lysozyme(LZM),superoxide dismutase(SOD),catalase(CAT),glutathione(GSH) were recorded to evaluate the immune capacities when exposed to different salinity changes: from 40 to 35,35 to 30,30 to 25,30 to 35 in 5 days,respectively.The results showed that the changing trends of 4 immune enzymes were almost the same.LZM activities decreased significantly and then increased gradually,finally remained above the control level(0 h).SOD activities significantly decreased and then remained at the control level.CAT activities decreased significantly and then increased,finally climbed to the control level.GSH activities increased significantly in a short time then increased significantly,finally reached the control level.The above results demonstrated that salinity change could significantly affect the immune parameters of scallop.
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