怀地黄脱毒试管苗培养条件的优化  

Optimization for Culture Condition of Virus-free Plantlets of Rehmannia glutinosa f.Hueichingensis(Chanet Sehih) Hsiao

作　　者：陈明霞[1,2] 周彩云[1] 李翔[3] 刘好书[1,4] 丁红娜[1] 唐果[1] 尚潇歌[1] 张晓丽[1,2] 李明军[1,2] 

机构地区：[1]河南师范大学生命科学学院,河南新乡453007 [2]河南省高校道地中药材保育及利用工程技术研究中心,河南新乡453007 [3]河南师范大学体育学院,河南新乡453007 [4]浚县实验中学,河南浚县456250

出　　处：《河南农业科学》2011年第5期128-132,共5页Journal of Henan Agricultural Sciences

基　　金：国家农业科技成果转化资金项目(2009GB2D000226);河南省教育厅科技攻关项目(2010A180009);河南师范大学青年科学基金(201qk14);河南师范大学大学生创新性实验项目(2008419)

摘　　要：研究了怀地黄脱毒试管苗在不同pH值、不同光照强度和不同温度下形态指标的差异,以寻求怀地黄脱毒试管苗的最适培养条件。结果表明：适合培养怀地黄脱毒试管苗的pH值在5.8~6.2,该pH值范围内,试管苗生长较健壮,株高和繁殖系数最大,分别为6.66 cm和4.8。最适合培养怀地黄脱毒试管苗的光照强度为2 500 lx,此光照强度下,试管苗生长较健壮,叶色浓绿,株高和繁殖系数分别为5.2 cm和4.6。最适合培养怀地黄脱毒试管苗的环境温度值为30℃,该温度下试管苗生长较健壮,株高和繁殖系数最高,分别为5.8 cm和4.8。因此,适合怀地黄脱毒试管苗生长的pH值为5.8~6.2、光照强度为2 500 lx、温度为30℃。In this paper,the morphological parameters of virus-free plantlets of Rehmannia glutinosa f.Hueichingensis（Chanet Sehih） Hsiao.were studied under different growing environment（pH value,different illumination intensity and different temperature） to find the optimum culture condition.The results were as follows：The optimum pH value was between 5.8 and 6.2,and the plantlets were healthy under pH5.8-6.2,the highest height and propagation coefficient were 6.66 cm and 4.8,respectively.The optimum illumination intensity was about 2 500 lx and plantlets were healthy under this illumination condition,the colour of leaves was deep green,plant height and propagation coefficient was 5.2 cm and 4.6,respectively.The best proper temperature was 30℃,and the plant height and propagation coefficient were 5.8 cm and 4.8,respectively.In a conclusion,the optimum condition for growth of virus-free plantlets of Rehmannia glutinosa f.Hueichingensis（Chanet Sehih） Hsiao.was：pH5.8-6.2,illumination intensity2 500 lx,and the proper temperature 30℃.

关 键 词：怀地黄 脱毒试管苗 PH 光照强度 温度 

分 类 号：S567.2[农业科学—中草药栽培]