压力对共培养系统中平滑肌细胞的增殖与刚度的影响  

Effect of Pressure on Proliferation and Rigidity of Airway Smooth Muscle Cells in Co-culture System

作　　者：邓林红[1] 赵国栋[1] 樊磊磊[1] 

机构地区：[1]重庆大学生物工程学院,重庆400044

出　　处：《贵州农业科学》2011年第5期156-159,163,共5页Guizhou Agricultural Sciences

基　　金：国家自然科学基金(30770529);重庆市自然科学基金计划重点项目(CSTC;2010BA5001);重庆大学大型仪器设备开放基金(2010063057)

摘　　要：为气道平滑肌细胞和气道上皮细胞之间的信息交流方式的研究提供借鉴与指导,先分别单独培养大鼠气道平滑肌和上皮细胞,然后建立以聚对苯二甲酸乙二醇酯(PET)膜为介质的气道上皮细胞与气道平滑肌细胞联合培养模型,用倒置显微镜、MTT法和原子力显微镜(AFM)对平滑肌细胞的生长情况和形态等研究。结果表明,在一定压力条件下,共培养模型中的气道上皮细胞对气道平滑肌细胞的增殖和刚度有促进作用。对弄清细胞之间的信号传递方式及进一步明白哮喘的病理原因具有重要意义。To provide reference and guidance for studying on exchange methods of information between airway smooth muscle cells and airway epithelial cells,the rat airway smooth muscle cells and epithelial cells were separately cultured first and then co-culture model of epithelial cells and smooth muscle cells was established by using polyethylene terephthalate（PET） membrane as medium.At last,the growth and morphology of smooth muscle cells on four different models were investigated by inverted microscope,MTT method and atomic force microscope（AFM）.The results show that under certain pressure,airway epithelial cells can promote the rigidity and proliferation of airway smooth muscle cells in the co-culture model,which is important to understand the signal way between cells and further study the pathology of asthma.

关 键 词：气道上皮细胞 气道平滑肌细胞 联合培养模型 压力 

分 类 号：S476[农业科学—农业昆虫与害虫防治]