水稻半矮秆基因iga-1的鉴定及精细定位  

Identification and Fine Mapping of a Semidwarf Gene iga-1 in Rice

作　　者：郭涛[1] 霍兴[1] 饶得花[1] 刘永柱[1] 张建国[1] 陈志强[1] 王慧[1] 

机构地区：[1]华南农业大学/国家植物航天育种工程技术研究中心,广东广州510642

出　　处：《作物学报》2011年第6期955-964,共10页Acta Agronomica Sinica

基　　金：国家自然科学基金项目(30771313);国家科技支撑计划项目(2008BAD97B02)资助

摘　　要：在前期通过空间诱变获得半矮秆隐性突变基因iga-1的基础上,进一步对iga-1进行鉴定。农艺性状调查表明携带iga-1的矮秆株系CHA-2、CHA-2N与原种特籼占13相比存在明显变异。节间长度测量显示CHA-2、CHA-2N节间比例正常,属dn型。外源GA3处理、内源GA3测定和α-淀粉酶活性检测揭示iga-1与GA3调控无关。利用CHA-2与粳稻品种02428杂交获得的F2群体将iga-1定位在水稻第5染色体两个InDel标记DL18和DL19间32.01kb的物理距离内。该区域有5个阅读框架,其中包括赤霉素信号传导调控基因D1。序列分析表明CHA-2、CHA-2N和特籼占13在D1位点上基因组序列不存在差异,推测D1并非iga-1的候选基因。比较水稻第5染色体上其他矮秆基因发现iga-1可能与半矮秆基因sd-7来自同一位点。A semidwarf gene iga-1 of rice （Oryza sativa L.） by mutagenesis of outer space treatment from Texianzhan 13 was identified.The dwarf lines CHA-2 and CHA-2N which carried iga-1 showed great variation in agronomic traits.On the basis of the internode length of CHA-2 and CHA-2N,the mutant belongs to the dn type of dwarfing.GA3 treatment,endo-GA3 measurement and α-amylase activity analysis in endosperm showed that iga-1 is independent of gibberellin acid.Using a large F2 population derived from a cross between the CHA-2 and an japonica rice variety,02428,the iga-1 gene was fine mapped into a 32.01 kb physical distance between two InDel markers,DL18 and DL19 on chromosome 5,where five open reading frames were predicted,one of which was the rice gibberellin-insensitive dwarf mutant gene D1.Sequence analysis showed that no variation in D1 locus was detected among CHA-2,CHA-2N and Texianzhan 13.Thus,D1 can not be the candidate gene of iga-1.Comparing the other dwarf genes on chromosome 5 showed that iga-1 is possibly allelic to the semidwarf gene sd-7.

关 键 词：水稻 半矮秆突变体 iga-1基因 精细定位 

分 类 号：S511[农业科学—作物学]