氯化两面针碱、白花丹素、紫杉醇的体外肝细胞毒性研究  

THE EXPERIMENTAL STUDY ON HEPATOCYTES TOXICITY OF NITIDINE CHLORIDE,PLUMBAGIN,TAXOL IN VITRO

作　　者：黄巨恩[1] 徐雅玲[2] 刘华钢[3] 

机构地区：[1]广西医科大学护理学院,南宁530021 [2]广西医科大学 [3]广西医科大学药学院

出　　处：《广西医科大学学报》2011年第2期192-195,共4页Journal of Guangxi Medical University

基　　金：国家自然科学基金资助项目(No.30850010)

摘　　要：目的:观察氯化两面针碱、白花丹素、紫杉醇在体外对肝细胞的毒性作用。方法:大鼠肝脏经胰蛋白酶和胶原酶消化,进行体外肝细胞培养。经糖原染色法(PAS)鉴定后,采用MTT法检测不同浓度氯化两面针碱、白花丹素、紫杉醇对肝细胞的毒性作用。结果:氯化两面针碱、白花丹素、紫杉醇对肝细胞IC50分别为(3.501 5±0.672),(13.289 1±1.165)和(2.163 9±0.748)mg/L,且最高抑制率均超过80%,从而说明氯化两面针碱、白花丹素和紫杉醇均对正常肝细胞有毒性作用。结论:氯化两面针碱、白花丹素、紫杉醇可抑制肝细胞的增殖,对肝细胞有一定的毒性。Objective：To detect the toxicity of nitidine chloride,plumbagin and taxol in rat＇s hepatocytes in vitro.Methods：SD rats＇ hepatocytes were cultured after digested with 0.25% trypsin,collagenase and identified by PAS.Hepatocytes proliferation was assayed by MTT method.Results：Treated with nitidine chloride,plumbagin and taxol,the 50 % inhibition concent-ration（IC50）of the hepatocytes was（3.501 5±0.672）,（13.289 1±1.165）,（2.163 9±0.748）mg/L;respectively and the biggest inhibitory rate over 80%,so nitidine chloride,plumbagin and taxol are cytotoxic in hepatocytes.Conclusion：Nitidine chloride,plumbagin and taxol have the function of inhibiting proliferation on the rats＇ hepatocytes.

关 键 词：氯化两面针碱 白花丹素 紫杉醇 肝细胞毒性 

分 类 号：R979.1[医药卫生—药品]