离体昆虫细胞对AcNPV和BmNPV的敏感性测定与分析  

Survey and Analysis for Susceptibility of in vitro Cultured Insect Cell Lines to AcNPV and BmNPV

作　　者：张欣[1] 冯颖[1] 丁伟峰[1] 马涛[1] 

机构地区：[1]中国林业科学研究院资源昆虫研究所国家林业局资源昆虫培育与利用重点实验室,云南昆明650224

出　　处：《林业科学研究》2011年第3期321-326,共6页Forest Research

基　　金：林业公益性行业科研专项(编号:4-38)

摘　　要：采用来源于3个目14种昆虫的离体细胞系对苜蓿银纹夜蛾核型多角体病毒AcNPV(Autographa californica nuclear polyhedrosis virus)和家蚕核型多角体病毒BmNPV(Bombyx mori nuclear polyhedrosis virus)进行敏感性测定。试验结果表明:各昆虫细胞系对两种病毒的敏感性不同,来源于双翅目及直翅目的细胞系均不能被这两种病毒感染;AcNPV可侵染来源于鳞翅目的Sf21、Sf9、HighFive、RIRI-PX及HZ,宿主范围较广;而BmNPV仅能侵染BmN细胞,宿主专一。AcNPV对Sf9细胞的感染率最高,接种病毒10 d后感染率平均达84.0%,而RIRI-PX的AcNPV病毒产量最高,形成的多角体数目为43.9PIBs/感染细胞。接种BmNPV10 d后,BmN的感染率为72.2%,形成的多角体数目为23.1.PIBs/感染细胞。Fourteen insect cell lines from three orders were used to be infected with Autographa californica nuclear polyhedrosis virus（AcNPV） and Bombyx mori nuclear polyhedrosis virus（BmNPV）.The result showed that the susceptibility for two viruses were different among different insect cell lines.The cell lines which come from Diptera and Orthoptera could not be infected with AcNPV and BmNPV.The five cell lines,Sf21,Sf9,HighFive,RIRI-PX and HZ,which come from Lepidoptera,could be infected with AcNPV.The BmN cell line could be infected only with BmNPV.The infection rate of AcNPV to Sf9 was the highest,reached 84.0% after 10 days infection.RIRI-PX infected with AcNPV could produce the highest yield of polyhedral inclusion bodies which reached 43.9 PIBs/cell at 10 day post infection.The infection rate of BmNPV to BmN was 72.2% after 10 days infection,and the yield of polyhedral inclusion bodies was 23.1PIBs/cell.

关 键 词：昆虫细胞系 ACNPV BMNPV 感染 

分 类 号：S763[农业科学—森林保护学]