一种新的白蜡虫寄生蜂体内Wolbachia的wsp基因分子检测及序列分析  

Molecular Detection and Sequence Analysis of wsp Gene from Wolbachia in a New Species of Ericerus pela Parasitic Wasps

作　　者：杨璞[1] 朱家颖[2] 谢正华[1] 李萌[1] 陈晓鸣[1] 陈晓庆[2] 

机构地区：[1]中国林业科学研究院资源昆虫研究所,国家林业局资源昆虫培育与利用重点实验室,云南昆明650224 [2]西南林业大学保护生物学院,云南省森林灾害预警与控制重点实验室,云南昆明650224

出　　处：《林业科学研究》2011年第3期340-344,共5页Forest Research

基　　金：国家自然基金委青年基金(31000983);云南省应用基础研究基金(2010ZC235);中国林科院资源昆虫研究所中央级公益性科研院所基本科研业务费专项资金(7-015-3)资助

摘　　要：采用Wolbachia的通用引物、A大组和B大组的特异性引物对一种新的白蜡虫寄生蜂——长尾啮小蜂(Apros-tocetus sp.)体内Wolbachia的wsp基因进行分子检测,所获得的基因片段分别命名为wApr、wAprA和wAprB,长度分别为620、566和463 bp;基因序列分析表明:wAprA、wAprB与wApr在对应位置序列仅有4个碱基的差异。系统发育分析表明:该寄生蜂仅感染了B大组Dig亚组的Wolbachia。wApr和wAprB基因序列已经递交到NCBI,登录号分别是HQ121415和HQ121417。PCR amplification was employed to detect the infection of Wolbachia on Aprostocetus sp.,a new species of parasitic wasps on Ericerus pela,by using the universal primers and specific primers for wsp gene of A and B supergroup.The sequences obtained,were named as wApr,wAprA and wAprB,which sizes were 620,566 and 463 bp respectively.Sequence analysis found that 4 variable basepairs occurred among the sequences.The phylogenetic analysis of wsp gene showed that Wolbachia in Aprostocetus sp.were classified as Dig group of B-Wolbachia.The sequences of wApr and wAprB were submitted to GenBank and the accession numbers assigned were HQ121415 and HQ121417.

关 键 词：白蜡虫 长尾啮小蜂 WOLBACHIA WSP 

分 类 号：S899.1[农业科学—特种经济动物饲养]