毒死蜱对舞毒蛾食物利用和解毒酶及AChE活性的影响  

Effects of Chlorpyrifos on Food Utilization and Detoxifying Enzymes and Acetylcholinesterase of Lymantria dispar

作　　者：李慧[1] 严善春[1,2] 王志英[1,2] 葛士林[1] 曹传旺[1,2] 

机构地区：[1]东北林业大学林学院森林保护学科,黑龙江哈尔滨150040 [2]东北林业大学林木遗传育种与生物技术教育部重点实验室,黑龙江哈尔滨150040

出　　处：《林业科学研究》2011年第3期345-349,共5页Forest Research

基　　金：国家973计划前期研究专项(2009CB125906);国家公益性行业科研项目(200904029-4);中国博士后基金(20090450942)

摘　　要：采用质量法和酶活性测定法研究了毒死蜱对舞毒蛾(Lymantria dispar)3龄幼虫食物利用的影响,并测定了其毒力及解毒酶、乙酰胆碱酯酶(AChE)的活性。用亚致死浓度(1.5 mg.L-1)毒死蜱处理小黑杨叶片,饲喂舞毒蛾3龄幼虫,其幼虫生长率(RGR)、食物利用率(ECI)和食物转化率(ECD)均显著低于对照,而近似消化率(AD)显著高于对照,相对取食量(RCR)处理和对照间差异不显著。毒死蜱对舞毒蛾幼虫24 h致死中浓度(LC50)为5.86 mg.L-1,其毒力低于三氟氯氰菊酯而高于氧化乐果。毒死蜱对舞毒蛾3龄幼虫体内羧酸酯酶(CarE)、谷胱甘肽S-转移酶(GSTs)和AChE均有抑制作用,抑制程度为CarE>AChE>GSTs。毒死蜱通过影响舞毒蛾食物利用和抑制酶活性而表现出杀虫活性,为一种有效防治舞毒蛾的有机磷杀虫剂。The effects of sublethal dose of chlorpyrifos（CPF） on food utilization of gypsy moth,Lymantria dispar,as well as CPF toxicity and enzyme activities of carboxylesterase（CarE）,glutathione S-transferases（GSTs） and acetylcholinesterase（AChE） were evaluated in the 3rd-instar gypsy moth larvae using gravimetric method and measuring enzyme activities.Relative growth rate（RGR）,efficiency of the conversion of ingested food（ECI） and efficiency of the conversion of digested food（ECD） of 3rd-instar larvae fed on poplar leaves treated by sublethal concentration of CPF were significantly lower than those fed on normal polar leaves.However,approximate digestibility（AD） of the treatment was significantly higher than that of control.The relative consumption rate（RCR） of the treatment and control groups were not significantly different.The 24 h LC50 of CPF to the gypsy moth 3rd-instar larvae was 5.86 mg·L-1,which was higher than that of omethoate but lower than that of cyhalothrin.In vitro inhibition assay indicated that the activities of AChE,CarE and GSTs were inhibited by CPF depended on concentrations.The enzyme activity inhibition percent is CarEAChEGSTs.The results suggest CPF is a kind of effective organophosphorous insecticide for controlling gypsy moth.

关 键 词：毒死蜱 舞毒蛾 食物利用 解毒酶 乙酰胆碱酯酶 

分 类 号：S763[农业科学—森林保护学]