油桐尺蠖核型多角体病毒(BusuNPV)生物学与检测技术研究  

Biological Activity of the BusuNPV and Its Detection Technology

作　　者：陈川[1] 张永安[1] 王玉珠[1] 曲良建[1] 林思诚 柯沛强 

机构地区：[1]中国林业科学研究院森林生态环境与保护研究所,国家林业局森林保护学重点实验室,北京100091 [2]广东省茂名市林业科学研究所,广东茂名525000

出　　处：《林业科学研究》2011年第3期410-414,共5页Forest Research

基　　金：林业公益行业专项基金(200704003)

摘　　要：油桐尺蠖（Buzura suppressartia Guenee）又称油桐尺蛾、大尺蠖,是一种暴食性害虫,主要危害茶、油桐、柑桔、杨梅、桉树等多种植物,常造成巨大的经济损失。国内主要分布在湖北、湖南、广东、广西等地区,国外在孟加拉西部以及印度地区有分布。目前对其采取的治理方法主要为化学农药防治,虽能起到一定的防效,却带来了污染环境,伤害天敌,生态环境平衡失调,降低茶叶、柑橘。Primers were designed according to sequence of polyhedron envelope protein gene（pep） of BusuNPV,then the polymerase chain reaction（PCR） technology was used to detect Buzura suppressaria nucleopolyhedrovirus DNA sequences from viral polyhedral inclusion bodies（PIB）,and the DNA sequences was detected from the egg and offspring.The level of sensitivity by the technology was as low as 1 fg·mL-1 DNA.Morphology and biological activity studies of BusuNPV showed that,the BusuNPV saved by laboratory was single-nucleocapsid nucleopolyhedrovirus（SNPV）.An infectivity test of BusuNPV against the second instar larvae of Buzura suppressaria was carried out in out laboratory,and LC50 of BusuNPV was determined as 6.65×104 PIB·mL-1,and LT50 of BusuNPV was 5.69 d.

关 键 词：BusuNPV 油桐尺蠖 PCR 生物活性 

分 类 号：S718.8[农业科学—林学]