120日龄金华猪骨骼肌酵母双杂交cDNA文库的构建  

Construction of yeast two-hybrid library of 120-day old Jinhua pig skeletal muscle

作　　者：陈哲[1] 沈一飞[1] 王颖[1] 徐宁迎[1] 

机构地区：[1]浙江大学动物科学学院,浙江杭州310029

出　　处：《浙江农业学报》2011年第3期511-513,共3页Acta Agriculturae Zhejiangensis

基　　金：国家高技术研究发展计划(2007AA10Z158);国家自然科学基金(30972078)

摘　　要：选用Clontech SMART技术,在酵母菌株中构建猪骨骼肌全长cDNA文库,文库容量约为1.6×10cfu,插入的双链cDNA片段的长度在500-2 250 bp,文库重组比率为93.75%。该文库可以用于进一步的酵母双杂交筛选。The whole-length cDNA library of 120-day old Jinhua pig skeletal muscle was constructed by the SMART technique.The titer of library was 1.6 × 106 cfu,the inserted cDNA fragment＇s length were between 500-2 250 bp,the recombination rate was 93.75%.The results showed that this library was good enough to be applied for the next step.

关 键 词：金华猪 骨骼肌 酵母双杂交 CDNA文库 

分 类 号：S828.82[农业科学—畜牧学]