毛花猕猴桃果实L-半乳糖内酯脱氢酶基因cDNA片段的克隆与序列分析  

Cloning and sequence analysis of cDNA fragments encoding L-galactono-1,4-lactone dehydrogenase from fruit of Actinidia eriantha

作　　者：燕标[1,2] 吴延军[2] 谢鸣[2] 叶爱华[1] 

机构地区：[1]安徽农业大学生命科学学院,安徽合肥230036 [2]浙江省农业科学院园艺研究所,浙江杭州310021

出　　处：《浙江农业学报》2011年第3期523-527,共5页Acta Agriculturae Zhejiangensis

基　　金：浙江省科技厅面上项目(2008C22007);浙江省自然科学基金(Y307030);宁波市农业成果转化项目(2008C30007);浙江省农业科学院院选项目

摘　　要：毛花猕猴桃果实中Vc含量极高,为了揭示毛花猕猴桃Vc形成规律,根据已知植物的L-半乳糖内酯脱氢酶(GalLDH)基因,设计简并引物,以毛花猕猴桃新品种‘华特’果实为材料提取总RNA,采用RT-PCR法,克隆出一条长661 bp的cDNA片段,根据测序结果推测其氨基酸序列,并与刺梨、拟南芥、甘薯和草莓等植物的GalLDH基因编码的氨基酸序列进行亲缘分析。结果表明,所克隆片段编码氨基酸序列与美味猕猴桃GalLDH相似度高达99%,其在同源基因进化关系上聚为一类,与葡萄的GalLDH相似性高达91%,与草莓、刺梨、甘薯、温州蜜柑、拟南芥、甜瓜等植物的GalLDH同源性均在80%以上,可知克隆到的核苷酸片段确为毛花猕猴桃GalLDH基因的cDNA片段。In order to investigate the molecular mechanism of Vc accumulation,661 bp cDNA encoding L-galactono-1,4-lacton dehydrognease（GalLDH） fragment was cloned from fruit of Actinidia eriantha by the method of RT-PCR,and then translated into amino acid sequences.In GenBank,compared with Actinidia delicisa,the amino acid sequence of GalLDH gene sequence showed 99% identity with those of Actinidia delicisa and they were both classified in the same family.The amino acid sequence of GalLDH gene sequence showed 91% identity with Vitis vinifera and more than 80% identity with Fragaria xananassa,Rosa roxburghii,Ipomoea batatas,citrur unshiu,Arabidopsis thaliana and Cucumis melo.These results suggested that the cDNA fragment cloned in this stady was from GalLDH gene of Actinidia eriantha.

关 键 词：毛花猕猴桃 L-半乳糖内酯脱氢酶 抗坏血酸 基因克隆 

分 类 号：S663.4[农业科学—果树学]