稻粒黑粉菌冬孢子萌发影响因素及其产孢培养基的筛选  

Factors influencing teliospore germination of Neovossia horrida and screening of sporulation medium of N.horrida

作　　者：陈胜军[1,2] 王艳丽[2] 张震[2] 姜华[2] 柴荣耀[2] 毛雪琴[2] 邱海萍[2] 王教瑜[2] 杜新法[2] 孙国昌[2] 

机构地区：[1]杭州师范大学生命与环境科学学院,浙江杭州310036 [2]浙江省植物有害生物防控重点实验室-省部共建国家重点实验室培育基地浙江省农业科学院植物保护与微生物研究所,浙江杭州310021

出　　处：《浙江农业学报》2011年第3期572-576,共5页Acta Agriculturae Zhejiangensis

基　　金：浙江省重大项目(2007C12905)

摘　　要：稻粒黑粉病是由担子菌亚门齿黑粉菌属的稻粒黑粉菌引起的。试验设置不同的温度、湿度、光照时间、pH值等培养参数,观察这些不同的参数对稻粒黑粉菌冬孢子萌发的影响。结果表明,冬孢子在充分吸水、温度24～28℃、光照与黑暗间隔培养12 h、pH值为7左右的条件下,最适宜冬孢子萌发。试验还观察并描述了稻粒黑粉菌冬孢子的形态特征和萌发过程。此外,为了建立稻粒黑粉病人工接种体系及研究稻粒黑粉菌与水稻之间的互作,需要筛选到能够产生稻粒黑粉病的接种体——次生小孢子的培养基。试验还研究了麸皮培养基等7种液体培养基的产孢效果,结果发现麸皮培养基最适合稻粒黑粉菌产生次生小孢子.Rice kernile smut is a kind of common disease which is caused by Neovossia horrida which belongs to Neovossia Kornicke genera,Basidiomycete ascomycotina subdivision.The main factors of affecting teliospore germination were investigated and the medium suitable for sporulation was screened in this article so that rice kernile smut can be prevented better.We set up different parameters in this research,for example,temperature,humidity,illumination time and pH value,and observed the influence of these different parameters on the germination of teliospore so that we could find out the most appropriate conditions that the teliospore of N.horrida could germinate.The results indicated that the optimal conditions of teliospore germination of N.horrida including appropriate temperature,adequate water etc.The light and dark interval for 12 hours was favorable for the germination of teliospores.The pH value equaling to seven and adequate water were optimal conditions for the teliospore germination.Meanwhile,the morphological characteristics and germination process of N.horrida were observed and described in this article.In addition,the suitable medium of sporulation was screened in order to establish artificial inoculation system of rice kernel smut and study the interaction between N.horrida and rice.We selected seven kinds of liquid media such as bran medium and investigated the sporulation effect of them.The results showed that the bran medium was the optimal liquid medium for production of the secondary sporidia of N.horrida.We found out the optimal condition under which the teliospore of N.horrida could germinate and screened the bran medium as the sporulation medium of N.horrida.

关 键 词：稻粒黑粉菌 冬孢子 萌发率 产孢培养基 

分 类 号：S435.11[农业科学—农业昆虫与害虫防治]