复方治障明眼液乙醇提取工艺研究  

Research on Alcohol Extracting Technology of Fu Fang Zhi Zhang Ming Eye-drop

作　　者：叶殷殷[1] 罗宪堂[1] 杨凤琼[1] 陈桂江[1] 

机构地区：[1]广东岭南职业技术学院,广东广州510663

出　　处：《临床医学工程》2011年第6期820-821,共2页Clinical Medicine & Engineering

摘　　要：目的研究复方治障明眼液乙醇提取工艺。方法以野黄芩苷、蒙花苷、大黄酚三种成分含量之和为考察指标,采用L9(34)正交试验筛选出最佳乙醇提取工艺,测定方法以HPLC法梯度洗脱为主。结果以三种成分之和计,正交试验各因素对实验结果影响大小为乙醇浓度>回流次数>回流时间>加醇量。结论复方治障明眼液乙醇提取最佳工艺为以70%乙醇8倍药量回流提取1小时,提取次数为3次。Objective To research the best alcohol extracting technology of fu fang zhi zhang ming eye-drop. Methods As the total contents of scutellarin,linarin and chrysophal to be evaluation indicator,adopted L9（34） orthogonal design to select out the best alcohol extraction. The main determination was by HPLC with gradient elution. Results Accounted by the total contents of the three kinds compositions,the influential effects of the experimental results were alcohol concentration reflux times the circumfluence time the volume of alcohol. Conclusion The best extracting technology is adding 8-fold times of 70% alcohol,percolating 1 hours,and three times.

关 键 词：醇提工艺 复方治障明眼液 野黄芩苷 蒙花苷 大黄酚 

分 类 号：R284.2[医药卫生—中药学]