ELISA法检测HBsAg实验条件的优化  

The optimized of ELISA examines HBsAg the experimental condition

作　　者：郭翀[1] 张真铭[1] 

机构地区：[1]昆明医学院附属第一医院检验科,650032

出　　处：《国际检验医学杂志》2011年第6期656-657,共2页International Journal of Laboratory Medicine

摘　　要：目的探讨温度、孵育时间、振荡对ELISA法检测HBsAg的影响,以期进一步优化检测条件。方法采用HBsAg含量约为1ng/mL的血清标本,在4种不同条件(37℃振荡、37℃不振荡、25℃室温震荡、25℃室温不振荡)下的3个不同反应时间(10、20、30min)测定HBsAg,记录吸光度值。结果除10min37℃不振荡和25℃不振荡差异无统计学意义(P>0.05)外,其余各组差异都有统计学意义(P<0.05)。结论 37℃振荡20min为ELISA检测HBsAg的较佳条件。Objective To analysis temperature,the incubating time,the vibration examine HBsAg to the influence of the ELISA method,further in expectation of optimizes the experimental conditions.Methods The HBsAg blood serum specimen were used,which density approximately was 1 ng/mL,in four kinds of different experimental conditions（37 ℃ vibration,37 ℃ no vibration,25 ℃ room temperatures vibrations,25 ℃ room temperatures no vibration） and three different reaction time（10 minutes,20 minutes,30 minutes）determine HBSAg,and register the numerical value of the absorbency.Results except 37 ℃ no vibration and 25 ℃ room temperatures no vibration exzist no apparent differences（P0.05）,they exzist the apparent differences（P0.05）.Conclusion 37 ℃ and vibrating 20 minutes is the best optimized condition of this experiment.

关 键 词：酶联免疫吸附测定 肝炎表面抗原 乙型 实验条件 

分 类 号：R446.6[医药卫生—诊断学]