树形对鲜黄梨果实糖积累及山梨醇转化相关酶活性的影响  

Effects of Tree Shape on the Sugar Accumulation and Activity of Sorbitol-Invertase of Pyrus pyrifolia ‘Sunhwang’

作　　者：汪志辉[1] 熊碧玲[1] 刘燕[1] 赵瑾 范娜娜[1] 蒋黎黎[1] 

机构地区：[1]四川农业大学园艺学院,雅安625014

出　　处：《林业科学》2011年第4期27-32,共6页Scientia Silvae Sinicae

基　　金：国家重点星火计划项目(2008GA810001)

摘　　要：以鲜黄梨为试材,应用WinsCanopy2005a冠层分析仪和光合测定仪对平棚形、V字形和疏散分层形3种树形的冠层结构特点和光合能力进行比较,测定果实发育过程中糖分含量及山梨醇转化酶活性,研究树形对果实中糖分积累及山梨醇转化相关酶活性的影响。结果表明:平棚形冠层开度和冠下直射、散射及总光合光量子通量密度显著高于其他2种树形,其透光性最好,光合能力最强。果实中可溶性总糖、葡萄糖、果糖、蔗糖、山梨醇的含量及山梨醇转化酶活性均以平棚形最高,V字形次之,疏散分层形最低且差异显著。果实在发育前期均以积累山梨醇和淀粉为主,后期以积累果糖为主;山梨醇脱氢酶和山梨醇氧化酶在果实发育早中期活性逐渐增加,后期二者活性降低,但较前期高。Pyrus pyrifolia ‘Sunhwang’ was used as experimental material.the canopy structure characteristics and photosynthetic capacity were comparatively investigated among the horizontal trellis system,V-shaped system and the sparse shaped canopy with WinsCanopy2005a and photosynthetic analyzer.The sugar content and activities of the sorbitol-invertase were measured during the fruit development stage to determined.It studied the effects of tree shape on the sugar accumulation process and activities of sorbitol-invertase in fruits.The results showed that canopy openness and photosynthetic photon flux density （PPFD） were significantly higher in Horizontal trellis system than that in the other two tree shapes.The light transmittance and photosynthetic capacity in Horizontal trellis system was the best among the three tree forms.Contents of the soluble sugar,starch,glucose,fructose,sucrose,sorbitol and the activities of sorbitol-invertase were the highest in the fruit of the level-shaped system,following by V-shaped system,and then the Sparse shaped canopy.The differences were significant.The accumulation sugars in the fruit mainly were starch and sorbitol at the early development stage and fructose at the late stage.The activities of SDH and SOX increased gradually in the fruit from early to middle development stage and decreased at the late stage but still were higher than that in prophase.

关 键 词：鲜黄梨 树形 糖积累 山梨醇 转化酶 

分 类 号：S718.43[农业科学—林学]