常用农药对5种昆虫细胞系的毒力测定及分析  

Measurement and Analysis of Cytotoxicity of 5 Insect Cell Lines Induced by Some Pesticides

作　　者：张欣[1,2] 冯颖[1,2] 丁伟峰[1,2] 马涛[1,2] 刘荣[2] 马艳[2] 

机构地区：[1]中国林业科学研究院资源昆虫研究所,昆明650224 [2]国家林业局资源昆虫培育与利用重点实验室,昆明650224

出　　处：《林业科学》2011年第4期182-189,I0001,I0002,共10页Scientia Silvae Sinicae

基　　金：林业公益性行业科研专项(4-38)

摘　　要：自20世纪60年代Grace建立了第一个能够稳定传代的昆虫细胞系以来(Grace,1962),经过几十年的研究和发展,昆虫细胞培养已在细胞系建立、培养技术和方法、昆虫细胞培养技术的利用等方面取得了很大的进展,昆虫细胞被广泛地应用于医学、农学及生物学的各个领域(宋德伟等,2004;张佑红等,2006;Maramorosch et al.,1992)。目前。The toxicity of lambda-cyhalothrin、beta-cypermethrin plus emulsion,E,C、alathion、azadirachtin and xylene at the different concentration and treating time was studied with five insect cell lines,applying Blackman viability analysis instrument.The results showed that the four types of pesticides had toxic effect on the cell lines,while xylene at three concentrations had no obvious toxic effect on the cell lines.The tests indicated that azadirachtin was significantly more toxic than other pesticides,then in turn were beta-cypermethrin plus emulsion,E,C,alathion,and lambda-cyhalothrin.The five cell lines responded differentially to the same testing concentration of same pesticide,and had different sensitivity to the chemicals.Amonge them,cell line Sf 9 was more sensitive than other cell lines,followed in turn by NIAS-MaBr-85,SL2,NIH-SaPe-4 and C6/36.The results indicated that the sensitivity of insect cell lines to the pesticides was in accord to the mechanism of pesticides,and the insect cell lines used for the determination of pesticide cytotoxicity is feasible.

关 键 词：毒力 昆虫细胞系 农药 二甲苯 致死率 

分 类 号：S718.7[农业科学—林学] S767