邓恩桉的组织培养  

Tissue Culture of Eucalyptus dunnii

作　　者：燕丽萍[1] 夏阳[1] 毛秀红[1] 刘翠兰[1] 王太明[1] 李丽[1] 李双云[1] 

机构地区：[1]山东省林业科学研究院,济南250014

出　　处：《林业科学》2011年第5期157-161,共5页Scientia Silvae Sinicae

基　　金：山东省农业良种工程项目(2006lz12-02);国家科技部成果转化项目(2006GB2C600167)

摘　　要：为了满足人们对木材与木质纤维的需求,桉树已成为南方工业原料林的主要造林树种,并不断北移扩大栽植。因大多数桉树不耐寒,限制了许多速生桉树的北移栽植。邓恩桉（Eucalyptus dunnii）原产澳大利亚,抗寒,速生,树干通直,木质粗细均匀,It is very difficult for Eucalyptus dunnii to produce roots in vitro.In order to resolve this problem,produce E.dunnii seedlings,and conduct gene engineering,an efficient and rapid micropropagation system by using seeds as explants was established for E.dunnii.The optimum medium for E.dunnii was obtained by modifying Mecown and Lloyd medium（WPM）,that is：with WPM large elements,plus Murashige and Skoog（MS） trace elements,MS Fe salt,Skirvin and Chu（SC） organic compounds and 30 g·L-1 glucose.This modified medium was used to resolve the serious problems,such as seedling brown and shoot tip death,in E.dunnii tissue culture.A modified WPM medium supplemented with 0.5 mg·L-1 6-benzy1aminopurine,0.5 mg·L-1 kinetin,0.2 mg·L-1 napthaleneacetic acid and 30 g·L-1 glucose was suitable for shoot induction and proliferation from divided buds,and the shoot proliferation rate of plantlets increased by 18.43 fold.The optimum growing medium was obtained by modifying WPM medium supplemented with 0.5 mg·L-1 KT,0.2 mg·L-1 TDZ and 0.01 mg·L-1 NAA.Over 84.3% regeneration plantlets were able to root after transferred to the modified half-strength MS medium supplemented with 3.0 mg·L-1 indole-3-butyric acid,0.01 mg·L-1 napthaleneacetic acid,0.05 mg·L-1 kinetin and 20 g·L-1 sucrose.When the micropropagated plantlets with well-developed root systems were transferred to planting bed containing a mixture of sand,soil and medium（4：1：1;V /V） in a greenhouse,93.8% of the plantlets survived.

关 键 词：邓恩桉 组织培养 微繁殖 

分 类 号：S723.13[农业科学—林木遗传育种] Q943.1[农业科学—林学]