基于纳米四氧化三铁的电化学生物传感器研究  

The Study of Electrochemical Biosensors Based on Fe_3O_4 Nanoparticles

作　　者：刘银环[1] 陈伟[1] 刘爱林[1] 朱丽君[1] 林新华[1] 

机构地区：[1]福建医科大学药学院药物分析学系,福州350004

出　　处：《福建医科大学学报》2011年第2期79-82,共4页Journal of Fujian Medical University

基　　金：国家自然科学基金(20805006);福建省医学创新课题(2009-CX-17);福建省自然科学基金(2010J06011);福建省高校产学合作重大项目(2010Y4003);福建医科大学重大科研项目(09ZD013)

摘　　要：目的构筑基于纳米四氧化三铁(Fe3O4)的电化学生物传感器,建立过氧化氢(H2O2)和模拟酶的电化学测定方法。方法利用纳米Fe3O4模拟过氧化物酶催化3,3’,5,5’-四甲基联苯胺(TMB)-H2O2的反应,采用传统的三电极体系,应用电流-时间曲线法检测酶催化产物。结果在最佳实验条件下,电流强度与H2O2浓度在0.5～6.0 mmol/L范围内呈线性关系,线性方程为:I(nA)=50.89c(mmol/L)+478.3,相关系数为0.994。电流强度与纳米Fe3O4的浓度在0.035～2.422 mmol/L范围内呈线性关系,线性方程为I(nA)=407.51c(mmol/L)+54.5,相关系数为0.996。结论纳米Fe3O4可催化H2O2氧化TMB产生电化学信号,该方法可应用于H2O2和纳米Fe3O4的含量测定。Objective The mimetic peroxidase property of iron oxide nanoparticles is studied by electrochemical method.An electrochemical biosensor is established and applied on the detection for the hydrogen peroxide and iron oxide nanoparticles.Method Iron oxide nanoparticles are used as mimetic peroxidase to catalyze the reaction between 3,3＇,5,5＇-tetramethylbenzidine（TMB） and H2O2.The enzyme-catalyzed product is detected by current-time curves.Results The linear response of current to the concentration of hydrogen peroxide ranging from 0.5 mmol/L to 6.0 mmol/L was obtained.The equation could be expressed as I（nA）=50.89c（mmol/L）＋478.3,with the correlation coefficient of 0.994.The linear response of current to the concentration of nanostructured Fe3O4 was also obtained.The equation could be expressed as I（nA）=407.51c（mmol/L）＋54.58,with the correlation coefficient of 0.996.Conclusion The Fe3O4 nanoparticle can catalyze oxidation TMB and create electrochemical signals.This method can be applied on the detection for hydrogen peroxide and iron oxide nanoparticles.

关 键 词：纳米结构 铁化合物 生物传感技术 电化学 催化作用 过氧化物酶 

分 类 号：R917[医药卫生—药物分析学]