人参皂甙Rh2提高人源肝癌细胞HepG2对桦木酸敏感性的作用机制  

Mechanism of Ginsenoside Rh2 in Enhancing Sensitivity of Human Liver Cancer HepG2 Cells to Betulinic Acid

作　　者：李清[1,2] 王晓宇[1] 李杨[1] 金英花[1] 

机构地区：[1]吉林大学分子酶学工程教育部重点实验室,长春130012 [2]大连大学生物工程学院,辽宁大连116622

出　　处：《中国生物制品学杂志》2011年第5期531-533,542,共4页Chinese Journal of Biologicals

基　　金：国家自然科学基金(90813003);大连市科技计划项目(2009E11SF135);吉林大学研究生创新基金资助项目(20111027)

摘　　要：目的探讨人参皂甙Rh2(G-Rh2)提高人源肝癌细胞HepG2对桦木酸(Bet A)敏感性的作用机制。方法将对数生长期的HepG2细胞分为4组:G-Rh2组(加入7.5μg/ml G-Rh2)、Bet A组(加入10μg/ml Bet A)、G-Rh2+Bet A组(加入7.5μg/ml G-Rh2和10μg/ml Bet A)及以不加药的细胞作为对照,荧光显微镜观察细胞形态变化,流式细胞术分析细胞凋亡,并检测细胞Caspase-3活性,Western blot检测细胞Caspase-3底物多聚(ADP-核糖)聚合酶[Poly(ADP-ribose)polymerase,PARP]断裂和Caspase-9激活情况。结果与G-Rh2和Bet A组相比,G-Rh2+Bet A组超过75%的细胞可见凋亡特征,且处于subG1期细胞的比例增加,Caspase-3活性增强,PARP和Caspase-9断裂明显。结论 G-Rh2提高HepG2细胞对Bet A的敏感性是通过细胞凋亡实现的,且涉及线粒体途径。Objective To investigate the mechanism of ginsenoside Rh2（G-Rh2） in enhancing the sensitivity of human liver cancer HepG2 cells to betulinic acid（Bet A）.Methods The HepG2 cells in logarithmic growth phase were divided into four groups.The cells in G-Rh2,Bet A and G-Rh2 ＋ Bet A groups were treated with 7.5 μg / ml G-Rh2,10 μg / ml Bet A and 7.5 μg / ml G-Rh2 ＋ 10 μg / ml Bet A respectively,while those in control group were untreated.The cells were observed for morphological change by fluorescent microscopy,for apoptosis by flow cytometry,determined for caspase-3 activity,then analyzed for breakage of poly（ADP-ribose） polymerase（PARP） and activation of caspase-9 by Western blot.Results Compared with those in G-Rh2 and Bet A groups,more than 75% of cells in G-Rh2 ＋ Bet A group showed the characteristics of apoptosis,in which the percentage of cells at subG1 stage and caspase-3 activity increased,while obvious breakages of PARP and caspase-9 were observed.Conclusion G-Rh2 enhanced the sensitivity of HepG2 cells to Bet A through mitochondrion-associated cell apoptosis.

关 键 词：人参皂甙RH2 肝肿瘤 桦木酸 药物协同作用 细胞凋亡 

分 类 号：R979.1[医药卫生—药品] R735.7[医药卫生—药学]