甜菜碱与蛋氨酸螯合铬对三江白猪血清生化指标及皮下脂肪组织脂肪酸合成酶基因mRNA表达的影响  

Effects of Betaine and Methionine Chelated Chromium (Ⅲ) on Serum Biochemical Indicators and Subcutaneous Fatty Acid Synthase mRNA Expression in Finishing Pigs

作　　者：崔波[1] 耿忠诚[1] 潘兴玲[1] 孙海涛[1] 刘胜军[1] 

机构地区：[1]黑龙江八一农垦大学动物科技学院,黑龙江大庆163319

出　　处：《中国生物制品学杂志》2011年第5期554-557,共4页Chinese Journal of Biologicals

基　　金：黑龙江省农垦总局攻关课题"肉猪规模化健康养殖关键技术的研究"(HNKXIV-08-03-01)

摘　　要：目的探讨甜菜碱与蛋氨酸螯合铬对三江白猪血清生化指标及皮下脂肪组织脂肪酸合成酶(Fatty acid synthase,FAS)基因mRNA表达的影响。方法选取81头平均体重约52 kg的三江白猪,采用3×3(蛋氨酸螯合铬×甜菜碱)二因子三水平有重复试验设计,将其分成9组,每组3个重复,每个重复3头猪。在玉米-豆粕型日粮基础上,添加不同水平的蛋氨酸螯合铬(以铬计,0、200、400μg/kg)和甜菜碱(0、1 000、1 250 mg/kg),研究二者对肥育猪血清生化指标及皮下脂肪组织FAS基因mRNA表达的影响。结果甜菜碱与蛋氨酸螯合铬均可降低肥育猪血清甘油三酯(TG)、总胆固醇(CHO)和胰岛素(INS)含量及FAS基因mRNA的表达水平,提高生长激素(GH)水平,复合添加9组血清TG浓度与对照组相比,差异最显著(P<0.05),并存在交互作用。复合添加6组血清CHO、GH和INS浓度与对照组相比,差异最显著(P<0.05),并存在交互作用,复合添加8组FAS基因mRNA的丰度与对照组相比,差异最显著(P<0.05),并存在交互作用。结论甜菜碱与蛋氨酸螯合铬均能减少脂肪沉积,且以复合添加效果较好,并存在一定的交互效应。Objective To investigate the effects of betaine and methionine chelated chromium（Ⅲ） on serum biochemical indicators and subcutaneous fatty acid synthase（FAS） mRNA expression in finishing pigs.Methods Eighty-one finishing pigs with a mean bodyweight of about 52 kg were divided into nine groups according to a 3 × 3 [methionine chelated chromium（Ⅲ） × betaine] test design with replicates at two factors and three levels.The methionine chelated chromium（Ⅲ）（0,200 and 400 μg / kg） and betaine（0,1 000 and 1 250 mg / kg） at various chromium levels were added into basal diet consisting of corn and bean dregs,and their effects on serum biochemical indicators and subcutaneous FAS mRNA expression in finishing pigs were evaluated.Results Both betaine and methionine chelated chromium（Ⅲ） decreased the serum triglyceride（TG）,total cholesterin（CHO） and insulin contents as well as expression level of FAS mRNA,and increased growth hormone（GH） level in finishing pigs.The TG content in group 9,CHO,GH and INS contents in group 6 as well as FAS mRNA level in group 8 showed the most significant differences with those in control group（each P 0.05）,and reciprocal effects were observed.Conclusion Both betaine and methionine chelated chromium（Ⅲ） decreased the fat deposition.However,the complex addition of betaine and methionine chelated chromium（Ⅲ） showed satisfactory decreasing effect and a certain reciprocal effect.

关 键 词：猪 甜菜碱 蛋氨酸螯合铬 脂肪酸合成酶复合物 脂肪代谢 

分 类 号：S816.4[农业科学—饲料科学] Q78[农业科学—畜牧学]