季节性流感裂解疫苗在小鼠中半数有效剂量ED50a的测定  

The 50 % effective dose （ ED50＂ ） of seasonal spilt influenza vaccine in mice

作　　者：黄保英[1] 王秀平[1] 谭文杰[1] 王文玲[1] 阮力[1] 

机构地区：[1]中国疾病预防控制中心病毒病预防控制所病毒病应急技术中心,北京10052

出　　处：《中华实验和临床病毒学杂志》2011年第2期92-95,共4页Chinese Journal of Experimental and Clinical Virology

基　　金：国家863计划资助（2006AA02A203）

摘　　要：目的探讨季节性流感疫苗在小鼠中的免疫原性及其使50％小鼠血凝抑制抗体滴度（HI）达到40的血凝素（HA）有效剂量（ED50。）。方法以我国2008—2009年度季节性流感裂解疫苗为模式，将该疫苗中针对甲型流感病毒HlNl与H3N2两种组分按照HA不同剂量免疫小鼠，通过比较1针免疫与2针免疫所产生的Hl抗体强度探讨季节性流感疫苗在小鼠中的免疫原性，确定疫苗免疫程序；此后，观察两种疫苗组分免疫小鼠后的HI抗体动力学，确定HI抗体产生的高峰期；最后，使用HA不同剂量免疫小鼠，在HI抗体高峰期测定使50％小鼠HI抗体滴度达到40的HA有效剂量（ED50。）。结果季节性流感疫苗1针与2针免疫结果显示，两种疫苗组分1针免疫可在小鼠中产生HI抗体滴度范围为10～120，2针免疫可以使HI抗体滴度为1针免疫的10～100倍；HI抗体动力学研究表明，两种疫苗组分1针免疫后第28～35天为HI抗体产生的高峰期；该高峰期的ED50‘测定结果表明，两种疫苗组分使50％小鼠HI抗体滴度达到40的HA有效剂量（ED50。）均为1．5μg。结论季节性流感疫苗在小鼠中具有良好的免疫原性，1针免疫后第28～35天为抗体产生的高峰期，使50％小鼠HI达到40的HA有效剂量为1．5μg，为建立以季节性流感疫苗为参考的免疫保护评价体系奠定了基础。Objective To evaluate the seasonal influenza spilt vaccine＇s immunogenicity and the 50% effective dose （ EDS0＂） of hemagglutin （HA） that can make 50% of the mice hemagglutination inhibition antibody （ HI ） titers to 40. Methods The 2008-2009 seasonal influenza spilt vaccine＇s two components, with HA from H1N1 and H3N2 influenza virus respectively, were used as a model. Mice were immunized once or twice with different doses, and the HI antibody titers were tested to determine the immunization procedure and to evaluate the immugenicity of seasonal influenza spilt vaccine in mice; Consequently, HI antibody response kinetics of the two components were observed to determine the time point when the HI antibody titer reached the peak point; Finally, mice were immunized with different doses of HA to evaluate the EDS0! that can make 50% of mice HI titers reach 40. Results Immunization procedures study showed that one-dose of seasonal influenza vaccine induced the HI antibody titers ranged from 10 to 120, while two-dose of influenza vaccine improved the HI antibody titer 10-100 times as compared with one dose ; antibody kinetics study suggested that the time point of HI antibody produced to peak is 28-35 days post one dose immunization; and the EDSO＂detection results indicated that one dose of 1.5 μg HA could make 50% of the mice HI antibody titer reach 40. Conclusion Seasonal influenza spilt vaccine is very immunogenic in mouse; the time point of HI antibody produced to peak is 28-35 days post one dose immunization; and the EDSOi of HA is 1.5 μg, which can make 50% of the mice HI titer reach 40. Theexperimental results provided foundation for the establishment of influenza vaccine evaluation system based on seasonal influenza vaccine.

关 键 词：流感疫苗 小鼠 血凝集抑制试验 

分 类 号：R392[医药卫生—免疫学]