中国部分甲肝病毒流行株结构蛋白VP3-VPl区基因特点分析  

Characterization of capsid protein VP3-VP1 genes of hepatitis A virus prevalent strains circulated in China

作　　者：郑惠惠[1] 曹经瑗[1] 毕胜利[1] 

机构地区：[1]中国疾病预防控制中心病毒病预防控制所,北京102206

出　　处：《中华实验和临床病毒学杂志》2011年第2期102-105,共4页Chinese Journal of Experimental and Clinical Virology

基　　金：国家自然科学基金项目（30671806）

摘　　要：目的分析中国部分甲肝病毒流行株结构蛋白VP3-VPl区基因特点。方法收集42份甲肝患者急性期血清标本，经核酸提取、逆转录及巢氏PCR，测得结构一非结构蛋白VP3-VPl-2A区序列，进行序列同源性比较并分析其基因特点。结果42株HAV病毒株在VPl-2A连接处核苷酸和氨基酸序列同源性分别为89．1％～100％和97．3％～100％；在全长结构蛋白VP3-VPl区的核苷酸和氨基酸序列同源性分别为87．6％～100％和98．8％～100％。VPl-2A连接处序列相同的病毒株在全长结构蛋白VP3．VPl区的核苷酸同源性为98．4％～100％，0～2个氨基酸位点不同。本实验所得序列在中和抗原位点处氨基酸序列均未变异。结论42株病毒株均属于I型，40株是IA亚型，2株IB亚型。本实验所用HAV流行株在结构蛋白VP3．VPl区的核苷酸存在差异但是氨基酸序列高度保守且没有中和抗原位点处的变异。VPl-2A结合处核苷酸序列相同的分离株在全长结构蛋白VP3-VPl区核苷酸序列相同或相近，氨基酸序列保守。Objective To analyse the genetic characteristics of the capsid protein VP3-VP1 region of hepatitis A virus strains circulated in China. Methods The nucleotide sequences of VP3-VP1-2A region of 42 HAV IgM positive serum samples were sequenced and analysed for nucleotide and amino acid identities and genetic characteristics of the VP3-VP1 region. Results The nucleotide and amino acid identities in the VP1-2A junction region among the 42 strains were 89.1% -100% and 97.3% -100% ; while in the complete VP3-VP1 region, the identities were 87.6%-100% and 98.8%-100%. Strains with identical nucleotide sequences in the VPI-2A junction region had 98.4%-100% nucleotide identity in the complete VP3-VP1 region and 0-2 amino acid differences in this region. There were no amino acid changes at neutralizing antigenetic sites of VP3-VP1 region within the 42 HAV strains. Conclusion All the 42 HAV strains belonged to genotype I,with 40 strains clustering to sub-genotype IA and 2 to sub-genotype IB. Different HAV strains analysed in this paper differed in the nucleotide sequences of the VP3-VP1 region ,but the amino acid sequences were highly conserved with no changes at neutralizing antigenetic sites. Both the nucleotide and amino acid sequences of the strains with the same VP1-2A junction region were identical or closely related when compared in the comnlete VP3-VPI region.

关 键 词：肝炎病毒 甲型 基因型 序列分析 

分 类 号：R51[医药卫生—内科学]