云南省乙型肝炎病毒基因型和血清型的流行状况分析  

Distribution of hepatitis B virus genotypes and serotypes in people who had a physical examination in Yunnan Province

作　　者：康文玉[1] 丁峥嵘[1] 沈立萍[2] 赵智娴[1] 田炳均[1] 李徽[1] 李勇[1] 张爽[2] 毕胜利[2] 

机构地区：[1]云南省疾病预防控制中心,昆明650022 [2]中国疾病预防控制中心病毒病预防控制所

出　　处：《中华实验和临床病毒学杂志》2011年第2期114-116,共3页Chinese Journal of Experimental and Clinical Virology

摘　　要：目的了解云南省人群中流行的乙型肝炎病毒（Hepatitis B virus，HBV）基因型和血清型分布情况。方法从参加健康体检的人群中筛查HBsAg阳性的血清标本，利用巢式PCR扩增HBVS基因片段，并对扩增产物进行序列测定，从GenBank中查获A～I基因型共27株HBV参考序列，构建HBVS基因系统发生树，以此确定其样本的基因型及血清型，并对结果进行统计分析。结果从2216名体检人员血清标本中筛查出39份HBsAg阳性的样本，HBV的s基因序列分型结果表明有4种基因型：c型占76．9％，B型占15．4％；D型占5．1％；I型占2．5％。血清分型结果为：adw2型占71．8％；adrq‘型占17．9％；ayr型占10．3％。所有adw2血清型标本均为c基因型。HBsAg、HBeAg双阳性标本中75％为c基因型／adw2血清亚型。结论云南省HBV感染人群中HBV基因型的分布以C型为主，血清型以adw2型为主。Objective To know genotypes and serotypes of hepatitis B virus（HBV） detected from hepatitis B infected people in Yunnan Province. Methods Serum samples were collected from HBsAg carriers detected from people who had a physical examination at Yunnan Provincial Center for Disease Control and Prevention. The S genes of HBV were amplified by nested PCR and the PCR products were sequenced. The viral genotype was identified by phylogenetic analysis. 27 reference sequences corresponding to HBV genotype A to I were obtained from GenBank. According to the amino acid sequences deduced from the nucleotide sequences of S gene, the dominant serotype of HBV detected from these people were confirmed. Results 39 HBsAg positive serum samples were detected from 2216 people who had a physical examination. The results shows that 76.9% were C genotype; 15.4% were B genotype; 5. 1% were D genotype;2. 5% were I genotype. Three serotypes were found. The rates of adw2, adrq＋ and ayr serotypes are 71.8% ,17.9% and 10. 3% respectively. All of adw2 subtype specimens are C genotype. Among the serum specimens in which both HBsAg and HBeAg are positive, 75% were C genotype and adw2 subtype. Conclusion It is determined that the main genotype and subtype of HBV prevailed in Yunnan province is C genotype and adw2 subtype.

关 键 词：肝炎病毒 乙型 序列分析 基因型 血清分型 

分 类 号：R181[医药卫生—流行病学] R51[医药卫生—公共卫生与预防医学]