复方瑞康欣可逆转吗啡戒断焦虑大鼠海马、杏仁核和伏核突触形态结构的可塑性  

Compound Ruikangxin can reverse the structural plasticity of synapses in hippocampus,amygdala and nucleus accumbens in morphine withdrawal rats

作　　者：何继锋[1] 钱刚[1] 凌锌[2] 吴明松[1] 郭萍[1] 罗素元[1] 

机构地区：[1]遵义医学院细胞生物学与遗传学教研室 [2]遵义医学院中心实验室,贵州遵义563000

出　　处：《解剖学报》2011年第3期300-306,共7页Acta Anatomica Sinica

基　　金：国家自然科学基金资助项目(30860373);教育部科学技术重点资助项目(206136);贵州省教委重点资助项目(黔教科2005109)

摘　　要：目的观察复方瑞康欣(CR)对吗啡戒断焦虑大鼠海马、杏仁核和伏核突触形态结构可塑性的影响。方法雄性SD大鼠84只,随机分为生理盐水对照组、吗啡戒断焦虑模型组、CR高、中、低剂量组和丁螺环酮组。以剂量递增方式皮下注射吗啡,10 d后自然戒断,于戒断1～3 d CR灌胃治疗后行高架十字迷宫实验。采用透射电镜技术比较各组(6只)突触体视学和界面结构各参数。结果在300 mg/kg和200 mg/kg CR组,大鼠进入开放臂次数的百分比和在开放臂停留时间的百分比明显增加(P<0.01或P<0.05);海马、杏仁核突触的数密度和面密度显著降低(P<0.01),突触连接带平均面积增加(P<0.01或P<0.05),伏核突触数密度降低(P<0.01);海马突触后致密物厚度、突触活性区长度、间隙宽度和界面曲率降低(P<0.01或P<0.05)。结论 CR能缓解吗啡依赖戒断所引起的焦虑,其细胞生物学机制可能是逆转吗啡戒断时海马、杏仁核和伏核突触形态结构的可塑性。Objective To observe effects on both anxiety-like behaviors and structural plasticity of hippocampus, amygdala and nucleus aecumbens in morphine withdrawal rats administrated with eolloeystis of compound Ruikangxiu （CR）. Methods SD rats, 84 in all, were randomly divided into control group, model group, CR groups involving high, middle and low dosages, and buspirone group. Gradually increasing dosages were applied to establish the model of morphine dependence in rats suffered from drugs （subcutaneous injection） for 10 days and followed by collocystis of CR （300, 200, and 100 mg/kg, intragastric administration） for 1-3 days. The elevated plus-maze tests were applied to validate the anxiety-like behavior in rats. Samples of hippocampus, amygdala and nucleus accumbens （n = 6） were harvested and further observed after preparation for electron microscope sample. Stereological methods and changeable parameters were applied to ensure accurate and unbiased comparisons of numerical density （Nv） , surface density （Sv） and average area of synaptic surface （S） in those samples. Results Compared with model group, higher percentage values of open arm entries and time spent in the open arms were observed in CR groups （300 and 200 mg/kg） （P 〈 0. 01, P 〈 0.05）. Lower scores of Nv and Sv in hippoeampus and amygdala （P〈0.01） and higher values of S （P〈0.01 or P〈 0. 05 ） were observed in those cured groups. Moreover, lower values of Nv in nucleus aeeumbens were detected （ P 〈 0.0t ） in the CR groups. Lower values of width of synaptic cleft, thickness of post synaptie density, length of active zones and curvature of synaptic interface were observed in CR （300 and 200 mg/kg） groups （P 〈 0.01 or P 〈0. 05）. Conclusion Obviously, collocystis of CR was capability of reversing the anxiety-like behavior in rats by rehabilitating structural plasticity of hippoeampus, amygdala and nucleus aecumbens, which could be the cell biological mechanism of anxious symptom.

关 键 词：复方瑞康欣 吗啡 戒断 突触 海马 杏仁核 伏核 透射电镜 大鼠 

分 类 号：R285.5[医药卫生—中药学]