X线辐射对仔鼠大脑颞叶皮层结构及乙酰胆碱酯酶活性、Bax蛋白和表皮生长因子表达的影响  

Effects of X-ray on the structure of cerebral temporal lobe cortex and activity of acetylcholinesterase and expresion of Bax and epidermal growth factor of filial mice

作　　者：王元春[1] 俞诗源[1] 王昱[1] 左文涛[1] 肖世南[1] 李丽[1] 

机构地区：[1]西北师范大学生命科学学院,兰州730070

出　　处：《解剖学报》2011年第3期307-313,共7页Acta Anatomica Sinica

基　　金：国家自然科学基金资助项目(30370163);甘肃省创新人才基金资助项目(ZS006-016)

摘　　要：目的探讨X线辐射对仔鼠大脑颞叶皮层结构及大脑乙酰胆碱酯酶(AChE)活性、颞叶Bax蛋白和表皮生长因子(EGF)表达的影响。方法对160只仔鼠(出生6～7d)用不同吸收剂量(0Gy、1Gy、3Gy、5Gy、7Gy)X线进行全身辐射,分别在辐射后1d、5d、10d和20d,用比色法检测仔鼠大脑皮层AChE活性的变化,用免疫组织化学方法检测颞叶皮层中Bax蛋白和EGF的表达,光学显微镜下观察仔鼠大脑颞叶皮层结构的变化。结果 X线辐射影响仔鼠大脑的生长发育。X线辐射仔鼠后,1Gy组在5d～20d时颞叶Bax蛋白阳性细胞数低于对照组,EGF阳性细胞数高于对照组,差异不显著(P>0.05),AChE活性高于对照组,差异显著(P<0.05);3Gy组EGF阳性细胞数和AChE活性先降低后升高,Bax蛋白阳性细胞数先增多后减少,差异显著(P<0.05);5Gy、7Gy组Bax蛋白和EGF呈强阳性表达,阳性细胞数高于对照组,AChE活性低于对照组,差异极显著(P<0.01)。辐射组仔鼠大脑颞叶皮层变薄、结构模糊,除1Gy组在1d时仔鼠大脑颞叶皮层第Ⅱ、Ⅲ、Ⅴ层,在5d时第Ⅰ、Ⅲ、Ⅳ、Ⅴ层及在20d时第Ⅱ、Ⅴ层的细胞面密度值高于对照组以外,其他辐射组颞叶皮层细胞密度值均低于对照组。结论 X线辐射影响了仔鼠大脑颞叶皮层的组织结构,这可能与大脑中AChE酶活性的降低及Bax蛋白、EGF表达的变化有一定关系。Objective To investigate effects of X-ray on the structure of cerebral temporal lobe cortex and acetylcholinesterase （ACHE） activity of the brain and expression of Bax protein and epidermal growth factor （EGF） of cerebral temporal lobe cortex in filial mice. Methods One hundred and sixty filial mice were irradiated with different dosages （0Gy, 1 Gy, 3Gy, 5Gy, 7Gy） of X-ray. The AChE activity was detected by colorimetry, expression of Bax protein and EGF of cerebral temporal lobe cortex were measured by immunohistochemistry, and the changes of cerebral temporal lobe cortex were observed by biomicroscopy. Results X-ray affected the development of cerebrum of filial mice. The number of Bax protein immunopositive cells in 1Gy group was lower and that of EGF was higher than that of the control group at the 5th-2Oth day after irradiation（ P 〉 0. 05 ） , whereas AChE activity was higher than that of the control group（ P 〈 0. 05 ）. The number of EGF immunopositive cells and AChE activity in 3Gy group firstly decreased and then increased（ P 〈 O. 05 ） , whereas that of Bax protein firstly increased then decreased （ P 〈 O. 05 ）. The expression of Bax protein and EGF was strongly positive in 5Gy and 7Gy groups,and the number of their positive cells was higher and AChE activity was lower than that of the control group（P 〈 0. 01 ）. The thickness of temporal lobe cortex decreased and their structure was not clear. Compared with the control group, cell density of temporal lobe cortex decreased except those in the cerebral temporal lobe cortex Ⅱ , Ⅲ , V layers at the 1st day and in Ⅰ ,Ⅲ, Ⅳ, V layers at the 5th day and in Ⅲ , V layers at the 20th day in 1Gy group. Conclusion X-ray radiation affected the structure of temporal lobe cortex in filial mice. This effect might becorrelated with the low activity of AChE and strongly positive expression of Bax protein and EGF in temporal lobe cortex.

关 键 词：X线 颞叶 乙酰胆碱酯酶 BAX蛋白 表皮生长因子 免疫组织化学 仔鼠 

分 类 号：Q954.4[生物学—动物学]