蛋白磷酸酶-2A催化亚基C在小鼠不同器官中的分化表达与定位  

Differential expression and distribution of the catalytic subunit C for protein phosphatase-2A in the different organs of mouse

作　　者：刘文彬[1] 惠珊珊[1] 胡小慧[1] 聂磊[1] 马慧[1] 王玲[1] 肖亚梅[1] 李万程[1,2,3] 

机构地区：[1]湖南师范大学生命科学学院蛋白质化学与发育生物学教育部重点实验室,长沙410081 [2]美国内布拉斯加州大学医学院生物化学与分子生物学系 [3]美国内布拉斯加州大学医学院眼科研究中心

出　　处：《解剖学报》2011年第3期384-388,共5页Acta Anatomica Sinica

基　　金：国家自然科学基金面上资助项目(30971658);湖南省芙蓉学者特聘教授基金资助项目(生080638);教育厅优秀青年资助项目(08B048)

摘　　要：目的探讨蛋白磷酸酶-2A(PP-2A)的催化亚基(PP-2Ac)在6只小白鼠不同器官中的表达模式与定位。方法运用RT-PCR、免疫印迹法及免疫荧光技术等实验手段,分别从mRNA水平、蛋白和组织水平进行研究。结果在mRNA水平上,PP-2Ac在大脑中表达最高,卵巢中表达次之,而肌肉、肾脏、脾脏、心脏、肝脏、肺及乳腺中表达相对较低,胃中表达最低;在蛋白水平上,也是大脑中的表达最高,卵巢、脾脏、心脏、肝脏和肺中表达水平相对较高,肌肉、肾脏、乳腺中表达相对较低,胃中表达最低。免疫荧光结果显示,PP-2Ac的表达具有一定的组织特异性和细胞特异性。结论 PP-2A是哺乳动物体内最重要的蛋白磷酸酶之一。Objective Protein serine/threonine phosphatase-2A （PP-2A） is one of the most important phosphatases in eukaryotes. To lay down a basis for exploring its physiological functions in mammals, we have analyzed the expression patterns and distribution of the catalytic subunits for PP-2A in the different organs of 6 adult mice. Methods RT-PCR,Western blotting analysis and immunofluorescence histochemistry were used to establish the expression patterns and distribution in various tissues of the adult mouse. Results RT-PCR revealed that the PP-2Ac mRNAs were expressed at the highest levels in the brain, at reduced levels in the ovary, at much reduced levels in the muscle, kidney, spleen, heart, liver, lung and breast, at the lowest levels in the stomach. Western blotting analysis demonstrated that the highest protein expression level of the PP-2Ac proteins was also detected in the brain. A reduced level of this protein was found in the ovary, spleen, heart, liver and lung. A much reduced PP-2Ae protein expression level was detected in the muscle, kidney and breast. And the lowest expression level of PP-2Ac was detected in the stomach. Immunohistochemistry analysis revealed the specificity of PP-2Ac distribution at both cellular levels and tissue levels. Conclusion Our results suggest that PP-2A is one of the most important protein phosphatases in mammalians.

关 键 词：蛋白质去磷酸化 蛋白磷酸酶-2A 催化亚基 信号转导 反转录-聚合酶链式反应 免疫印迹法 免疫荧光 小鼠 

分 类 号：Q78[生物学—分子生物学]