何首乌饮对过度训练大鼠睾丸组织3β-羟基类固醇脱氢酶的影响  

Effect of Heshouwuyin on the expression of 3β-hydroxysteroid dehydrogenase protein in testis tissue of overtrained rats

作　　者：王凤威[1] 曲银娥[2] 赵秀军[3] 牛嗣云[4] 孙一翀[1] 陈雪[5] 郭凯华[6] 高福禄[7] 

机构地区：[1]承德医学院基础医学部,河北承德067000 [2]河北联合大学医学部组织学与胚胎学教研室,河北唐山063009 [3]河北医科大学组织学与胚胎学教研室,石家庄050017 [4]河北大学医学部组织学与胚胎学教研室,河北保定071000 [5]河北医科大学研究生院,石家庄050017 [6]中国人民解放军第260医院妇产科,石家庄050041 [7]河北师范大学,石家庄050016

出　　处：《解剖学报》2011年第3期394-398,共5页Acta Anatomica Sinica

基　　金：河北省自然科学基金资助项目(C2009000269)

摘　　要：目的探讨何首乌饮对过度训练大鼠睾丸组织3β羟基类固醇脱氢酶(3β-HSD)的影响。方法 30只雄性SD大鼠随机分为5组,A组为安静对照组;B组为安静何首乌饮组;C组为自然恢复组;D组为何首乌饮治疗组;E组为何首乌饮预防组,每组6只。C组、D和E组大鼠复制力竭运动动物模型,其中的E组每天训练前给何首乌饮20g/(kg.d)(含生药9.6kg/L)灌胃作为预防。模型成功后D组给何首乌饮20g/(kg.d)(含生药9.6kg/L)灌胃治疗60d。放射免疫法检测各组血清睾酮浓度,分别采用RT-PCR、Western blotting和免疫组织化学法检测各组睾酮合成催化酶3β-HSD的表达变化。结果 3β-HSD蛋白的阳性信号为间质细胞的胞质表达棕黄色颗粒。3β-HSD在组织、蛋白和mRNA的表达变化:B组明显高于A组(P<0.05),E组和D组高于C组(P<0.05),E组和D组之间无显著性差异。结论何首乌饮可以增强睾酮合成限速酶3β-HSD的表达。Objective To explore the effect of Heshouwuyin （HSWY） on the expression of 3β-hydroxysteroid dehydrogenase （3β-HSD） protein in testis tissue of overtrained rats. Methods Thirty SD rats were randomized into 5 groups, including normal control group （ A group） , HSWY administered normal group （ B group）, model control group （ C group） , HSWY treated group （D group） , HSWY beforehand-prevented group （E group） , 6 animals in each group. The model of C, D and E group were made by overtraining, the D group was treated with HSWY by intragastric administration for 60 days after the success of model[20g/（kg·d） including crude drug 9.6kg/L], and the E group was prevented by administering HSWY before overtraining[ 20g/（ kg· d） including crude drug 9.6kg/L]. The density of testosterone was detected by radio-immunity, the expression of 313-HSD was measured by immunohistochemistry, Western blotting and RT-PCR. Results Compared with C group, 3β-HSD protein was expressed in plasmid of leydig cells, and the expressions of313-HSD and 3β-HSD mRNA in D group and E group were increased（P 〈0. 05） with no difference between D group and E group. Compared with A group, the expressions of 3β-HSD protein and 3β-HSD mRNA in B group increased（P 〈 0. 05）. Conclusion Heshouwuyin can promote the expressions of 3β-HSD.

关 键 词：何首乌饮 过度训练 睾酮 3B羟基类固醇脱氢酶 免疫印迹法 免疫组织化学 大鼠 

分 类 号：R339.4[医药卫生—人体生理学]