大鼠小肠壁连续切片的三维重建  

Three-dimensional reconstruction of rat small intestine

作　　者：杜鹃[1] 曹雷[1] 刘锋[1] 任国山[1] 周宇宁[1] 李伟[1] 崔慧先[1] 

机构地区：[1]河北医科大学人体解剖学教研室,石家庄050017

出　　处：《解剖学报》2011年第3期421-424,共4页Acta Anatomica Sinica

摘　　要：目的在肠壁、微绒毛及肠腺3个观察水平对大鼠小肠进行三维重建,观察其内部各结构的三维形态学特征及空间位置关系。方法正常SD大鼠1只,取小肠制作石蜡包埋标本,经连续切片、HE染色后,进行数字化图像采集;经图像拼接、预处理、配准、分割及三维重建等步骤后,观察各结构空间相互位置关系,并进行形态学指标测量。结果三维重建图像能够直观、立体地显示肠壁及肠壁内各结构(如动脉、静脉、淋巴管、微绒毛、肠腺等)的三维形态及相互空间位置关系。根据重建结果测量得到多项形态学指标,如微绒毛密度、平均高度,肠腺密度、体积。结论小肠黏膜下层与微绒毛内部存在丰富的血管网及淋巴管网,其排列方式与小肠的吸收及免疫功能密切相关。Objective To observe the three-dimensional morphological characteristics and spatial relationship between the components of rat small intestine based on three different observations： intestinal wall, microvillus and enteraden. Methods A normal SD rat, take the small intestine to produce paraffin specimens. After the processes of serial section and HE staining, digitalized images were carried out in different objective rates. After the processes of assembling,preprocessing,alignment, partition and three-dimensional reconstruction, the spatial relationship between each structure was observed and morphological indexes were measured. Results The three-dimensional configuration and the spatial position of intestinal wall and its internal structures （ arteriovenous, lymphatic, microvillus, enteraden, ete） could be displaied intuitirely. Based on the reconstructed image results, some morphological features were measured, which were microvillus density and average height, intestinal density and volume. Conclusion Small intestine submucosa and internal mierovillus have abundant vascular nets and lymphatic pipelines, their arrangements are closely related to absorb and immune function of small intestine.

关 键 词：小肠 微绒毛 肠腺 连续切片 三维重建 大鼠 

分 类 号：R319[医药卫生—基础医学]