小型猪经脉区皮肤微血管的三维重建  

Three-dimensional reconstruction of mini-pig skin microvasculars in meridlans

作　　者：吕钊君[1] 张涛[2] 郭洋[2,3,4] 薛久洲[1] 李华[5] 胡平[5] 穆祥[2] 

机构地区：[1]北京农学院动物科学技术系,北京102206 [2]北京农学院兽医学(中医药)北京市重点实验室,北京102206 [3]沈阳农业大学畜牧兽医学院,沈阳110161 [4]吉林大学畜牧兽医学院,长春130062 [5]中国科学院计算技术研究所,北京100190

出　　处：《解剖学报》2011年第3期425-428,共4页Acta Anatomica Sinica

基　　金：北京市属高等学校人才强教计划资助项目(PHR201007142)

摘　　要：目的利用小型猪后肢膀胱经经脉线区皮肤连续组织切片,通过三维重建软件构筑后肢膀胱经经脉线区微血管三维图像。方法 3月龄中国农业大学Ⅲ系小型猪,经络定位仪定位后肢膀胱经。在第3腰椎与荐骨之间取1.2cm×0.5cm×0.5cm中间标有经脉线的皮肤。在取材皮肤的经脉线正下方真皮网状层与皮下组织交界处(距离表皮层约1mm),用刀片开一"V"型凹槽作为定位标记,制作经脉线皮肤垂直切面连续切片(厚10μm),每隔1张取1张,共取500张,进行微血管免疫组织化学显色。以定位标记为参照,采集二维图像,使用Neuro软件进行三维重建。结果构筑出的经脉线区微血管的图像具有立体感,可任意剖割并从不同方向显示其内部微血管构筑情况。结论利用小型猪经脉线区皮肤免疫组织化学微血管显色的连续组织切片,结合计算机三维重建技术,能够进行经脉线区皮内微血管的三维构筑,并可从任意角度观察。Objective To make three-dimensional picture reconstruction of microvasculars in bladder meridian of hindlimb area by using serial skin tissue sections of bladder meridian of hindlimb in mini-pig and the tool software. Methods Three-month-old mini-pig of China Agricultural University m series, positioning the bladder meriadian. To obtain the skin tissue sanples with the volume of 1.2cm× 0. 5cm × 0. 5cm between the third lumbar and sacrum, which was marked with meridian line in the center. Then cutting a ＂V＂ type groove as alignment mark at the juncture of dermis reticular layer and subcutaneous tissue under the meridian line of sample （lmm distance to epidermis） by using scalpel. Vertical-sectional serial sections of skin （101μm thick） were made, 500 tussise sections were collected from every other piece of tussise sections, then the microvasculars were stained by the means of immunohistochemistry. Two-dimensional pictures were collected with microscrope by the direction of positioning mark, then three-dimensional pictures were constructed. Results The constructed microvasculars in bladder meridian of hindlimb area were tridimensional observed with naked eye. The three dimensional picture could be splitted optional and showed the construction of microvasculars in different directions. Conclusion The microvasculars in bladder meridian of hindlimb area can be reconstructed and observed from any angle by using the method of serial skin tissue sections and microvascular staining by immunohistochemisity, as well as the technique of computerized three dimensional reconstruction.

关 键 词：微血管构筑 经脉线 免疫组织化学 三维重建 猪 

分 类 号：R319[医药卫生—基础医学] R322.12