NR2F2促进小鼠MC3T3-E1前成骨细胞增殖  

NR2F2accelerates mouse MC3T3-E1 pre-osteoblast proliferation

作　　者：王崧[1,2] 熊霞辉[1,2] 朱宁[1,2] 陈梅红[1,2] 

机构地区：[1]中国医学科学院基础医学研究所 [2]北京协和医学院基础医学院生物化学与分子生物学系医学分子生物学国家重点实验室,北京100005

出　　处：《基础医学与临床》2011年第6期647-651,共5页Basic and Clinical Medicine

基　　金：国家自然科学基金(90608023);国家(重点)实验室专项经费(2060204)

摘　　要：目的探讨NR2F2基因对小鼠前成骨细胞增殖的影响。方法用实时定量PCR检测mRNA表达量,MTS法检测细胞增殖,流式细胞术检测细胞周期,ELISA-Brdu法检测细胞DNA合成速度。结果在小鼠MC3T3-E1前成骨细胞增殖速度加快时,NR2F2基因的表达增高至对照的4.57±0.30倍(P<0.01)。过表达NR2F2基因促使MC3T3-E1细胞增殖速度加快,细胞数量增加(P<0.01),细胞周期中S期细胞比例明显升高,为对照组的2倍,G2/M期比例也有增加(P<0.05)。过表达NR2F2基因使MC3T3-E1细胞的Brdu掺入率增高,DNA的合成加速(P<0.01)。结论 NR2F2使S期细胞比例增加,促进小鼠前成骨细胞MC3T3-E1的增殖速度。Objective To study the role of NR2F2 in murine pre-osteoblast proliferation.Methods Quantitative real-time PCR was used to measure the mRNA level,MTS assay was used to assess the cell proliferation rate,flow cytometry was used to figure out the cell cycle distribution,and ELISA-Brdu was used to check the rate of DNA synthesis.Results When murine pre-osteoblast MC3T3-E1 cell proliferation was accelerated,the expression level of NR2F2 remarkably increased to 4.57±0.30（P0.01） fold compared with that of the control.Over-expression of NR2F2 accelerated MC3T3-E1 cell proliferation（P0.01）,and the proportion of cells in S phase markedly increased to 2 fold compared with that of the control,and the proportion of cells in G2/M phase also went up（P0.05）.More Brdu was incorporated into NR2F2 over-expressing cells,indicating the increase of DNA replication rate（P0.01）.Conclusion NR2F2 accelerates murine pre-osteoblast MC3T3-E1 proliferation through increasing the proportion of cells in S phase.

关 键 词：NR2F2 MC3T3-E1细胞 细胞增殖 

分 类 号：R346[医药卫生—基础医学]