线粒体分裂对胰岛β细胞功能的影响  

Effects of mitochondrial fission on the function of pancreatic β cells

作　　者：门秀丽[1] 张文健 许世清 徐梅 徐雅平 娄晋宁[1] 

机构地区：[1]河北联合大学病理生理学教研室,唐山063000 [2]日友好医院临床医学研究所

出　　处：《中华内分泌代谢杂志》2011年第5期422-426,共5页Chinese Journal of Endocrinology and Metabolism

基　　金：国家基础研究基金（2006CB503906）;国家自然科学基金（30971410）

摘　　要：目的探讨线粒体分裂对胰岛B细胞功能的影响。方法通过可诱导表达野生型动力相关蛋白1（Drp-1WT）基因和突变型Drp-1（Drp-1K38A）基因的大鼠INS-1β细胞，分析不同葡萄糖条件下，线粒体分裂对胰岛β细胞功能的影响。结果在高糖条件下，强力霉素诱导后的Drp-1WT细胞线粒体分裂过程增强，网络状结构部分断裂，多呈点状结构，细胞胰岛素分泌功能降低（P〈0．01），线粒体膜电位降低（P〈0．05），细胞内ATP含量减少（P〈0．05），细胞色素C表达增加，而在Drp-1K38A细胞中，上述变化明显减轻。结论线粒体分裂增强可抑制胰岛β细胞功能。Objective To investigate the effect of mitochondrial fission on the function of pancreatic β cells. Methods INS-1 stable cell lines allowing inducible expression of either wild-type dynamin-related protein 1 （Drp-1WT） or its dominant-negative mutant （Drp-1K38A） were used. The effect of mitochondrial fission on the function of pancreatic β cells were investigated under different concentrations of glucose. Results There were increased mitochondrial fission and disintegration of the mitochondrial reticulum into multiple punctiform organelles in Drp-1WT cells induced with doxycycfine under high glucose condition. Insulin secretion （ P〈0.01 ） , mitoehondrial membrane potential （P〈0.05）, and ATP content （P〈0.05）were decreased and eytochrome C expression was increased after the expression of Drp-1WT under high glucose condition while these changes were markedly mild in Drp-1K38A expression cells. Conclusion The increased mitochondrial fission inhibits pancreatic β cell function.

关 键 词：线粒体分裂 动力相关蛋白1 Β细胞功能 ATP 

分 类 号：R587.1[医药卫生—内分泌]