鸡核受体辅激活蛋白1基因的荧光定量PCR检测  

Real-time fluorescent quantitative PCR assay for chicken nuclear receptor co-activator 1 gene

作　　者：赵振华[1] 黎寿丰[1] 黄华云[1] 李春苗[1] 张静[1] 薛龙岗[1] 丁余荣[1] 

机构地区：[1]中国农业科学院家禽研究所,江苏扬州225003

出　　处：《江苏农业学报》2011年第3期577-580,共4页Jiangsu Journal of Agricultural Sciences

基　　金：江苏省"333"工程培养基金项目(BRA2010162);中国农业科学院家禽研究所博士基金项目(JQ2009002);现代农业产生技术体系建设专项基金项目

摘　　要：鸡核受体辅激活蛋白1基因(NCOA1)mRNA的表达对性腺的发育、成熟均起关键作用,并且与繁殖性状相关。为了建立荧光定量PCR技术检测鸡NCOA1表达量的方法,根据GenBank中NCOA1基因序列设计合成了引物,以β-action基因为内参基因,对荧光定量PCR方法进行了评估。结果表明:构建的标准曲线线性关系良好,建立的NCOA1基因荧光定量PCR检测方法灵敏度高、特异性强,准确可靠。建立的SYBR Green I实时荧光定量PCR方法可以测定鸡NCOA1mRNA表达水平的相对含量。The expression of nuclear receptor co-activator 1（NCOA1） of chicken mRNA is key to development and maturity of gonads,and is related to reproduction performance.The aim of this study was to establish a fluorescent quantitative PCR（FQ-PCR） method for NCOA1 expression.The primer were designed and synthesized according to the NCOA1 sequence of chicken available in GenBank.The real-time RT-PCR assay was developed and assessed by reference gene of β-action.Results showed that the standard curve had good linear dependence,and the fluorescent quantitative RT-PCR assay was sensitive,specific and reliable.The results indicated that the relative expression of NCOA1 mRNA could be detected using the real-time PCR with SYBR Green I.

关 键 词：鸡 核受体辅激活蛋白1基因 实时荧光定量PCR 

分 类 号：S831.2[农业科学—畜牧学]