灵芝多糖肽拮抗吗啡的免疫抑制作用的体外试验  

ANTAGONISTIC EFFECT OF GANODERMA POLYSACCHARIDE PEPTIDE AGAINST IMMUNOSUPPRESSION OF MORPHINE IN VITRO

作　　者：陆正武[1] 林志彬[1] 

机构地区：[1]北京医科大学基础学院药理学系,北京100083

出　　处：《中国药物依赖性杂志》1999年第4期260-262,共3页Chinese Journal of Drug Dependence

摘　　要：目的··:研究灵芝多糖肽(GPP)对吗啡所致免疫抑制的拮抗效应。方法··:采用中性红法检测小鼠腹腔巨噬细胞吞噬功能;采用[3H]TdR参入法测定淋巴细胞增殖反应;分别采用胸腺细胞增殖法和依赖IL-2和HT-2细胞株测定IL-1和IL-2生成。结果··:体外GPP50-800μg·ml-1可以不同程度地翻转高浓度吗啡所致的巨噬细胞吞噬功能降低,IL-1/IL-2产生减少及淋巴细胞增殖功能减退,使之恢复正常。结论··:提示GPP直接地使遭受抑制的免疫细胞的功能得以恢复,产生功能性拮抗作用。Objective:To investigate the antagonistic effects of ganoderma polysaccharide peptide (GPP) against immunosuppression of morphine.Method:Phagocytosis of mouse peritoneal macrophages was determined by neutral red method;lymphocyte proliferation induced by Con A was measured by TdR incorporation;IL-1/IL-2 production was assayed by thymocyte proliferation and IL-2 dependent HT-2 cell line respectively.Result:GPP 50-800 μg·ml-1 differently blocked suppressive effects of high doses of morphine on macrophage phagocytosis,IL-1/IL-2 activity,and splenocyte mitogenity to Con A.Conclusion:GPP may directly activate immune cells to improve immune functions depressed by in vitro treatment of morphine.

关 键 词：灵芝多糖肽 吗啡 巨噬细胞 淋巴细胞增殖 

分 类 号：R285.5[医药卫生—中药学] R971.2[医药卫生—中医学]