不同部位阻断RAAS对心肌梗死大鼠胶原重构的影响  

Effects of Blockage of the Renin-Angiotensin-Aldosterone System at Different Sites on Collagen Remodeling in Myocardial Infarction Rats

作　　者：任谦[1] 孙根义[2] 张世姝[2] 刘志勇[2] 毛用敏[2] 

机构地区：[1]浙江省杭州市第三人民医院心内科,浙江杭州310009 [2]天津胸科医院心内科,天津300051

出　　处：《中国现代医生》2011年第18期24-26,41,共4页China Modern Doctor

摘　　要：目的比较不同部位阻断肾素-血管紧张素-醛固酮系统(RAAS)对心肌梗死(MI)大鼠心肌胶原重构的影响。方法将MI术后24h存活的Wistar大鼠随机分配至梗死组(MI组)、西拉普利组(ACEI组)、缬沙坦组(ARB组)和螺内酯组(SPI组),假手术组(SHAM)作为对照。2周后,测量各组大鼠体重(BW)、心脏重量(HW)、心脏重量指数(HWI),放射免疫法测定血浆AngⅡ、醛固酮(Ald)含量,逆转录-聚合酶链(RT-PCR)方法测定心肌Ⅰ型胶原、Ⅲ型胶原mRNA表达。结果 ①与SHAM组比较,MI组HW、HWI,血浆AngⅡ、Ald含量,心肌Ⅰ型胶原、Ⅲ型胶原mRNA表达均显著增加(P<0.01);②与MI组比较,除ARB组血浆AngⅡ、SPI组血浆Ald含量显著升高外(P<0.01),各治疗组HW、HWI、血浆AngⅡ、Ald含量、心肌Ⅰ型胶原、Ⅲ型胶原mRNA表达均显著下降(P<0.05);③除ARB组血浆AngⅡ、SPI组血浆Ald含量显著升高,SPI组梗塞区心肌Ⅲ型胶原mRNA表达显著下降外(P<0.01),各治疗组其余各指标均无差异(P>0.05);④MI组及各治疗组内梗塞区心肌胶原mRNA表达均较非梗塞区显著增加(P<0.05)。结论 MI后AngⅡ、Ald激活共同促进胶原合成,西拉普利、缬沙坦、螺内酯从不同部位阻断RAAS均可抑制梗塞后胶原重构。Objective To investigate the effects of blockage of the Renin-Angiotensin-Aldosterone system at different sites on the after rats myocardial infarction. Methods Twenty-four hours after MI model was established by ligation of the left anterior descending coronary artery,Wistar rats were randomized devided into untreated group （MI） ,ACEI group ARB group and SPI treated group for 2 weeks. Sham-operated rats served as controls （SHAM）. The weight of rats body and heart were measured. The level of plasma Ang Ⅱ and aldosterone were examined by radioimmunoassay. The mRNA level of cardiac collagen type Ⅰ and Ⅱ were measured by reverse-transcription-polymerase chain reaction（RT-PCR）. Results MI enhanced heart weight （HW）,heart weight index（HWl）,plasma Ang Ⅱ level and aldosterone,expression of the mRNA level of collagen type Ⅰ and Ⅲ were increased compared with sham-operated group with a significant difference （P〈0.01）. Treated with Cilazapril,Valsartan and Sipironolactone prevented the MI-induced rise in HW, HWI significantly （P〈0.01）. All treatment prevented the rise of cardiac collagen gene expression significantly （P〈0.01）, and to the normal in the noninfarct zone （P〉0.05）,but not in the infarction zone （P〈0.01）. Conclusion MI was associated with an increase in plasma Ang 11 and aldosterone,wbich might be involved in post-infarcted ventricular remodeling. Treated with Cilazapril,Valsartan and Sipironolactone had additive effect in attenuating the remodeling of cardial collagen after myocardial infarction.

关 键 词：心肌梗死 胶原 血管紧张素转换酶抑制剂 血管紧张素Ⅱ1型受体拮抗剂 醛固酮拮抗剂 

分 类 号：R542.22[医药卫生—心血管疾病]