胃癌中REIC蛋白分泌水平及其增殖抑制作用研究  

The secreted level of REIC protein in gastric carcinoma and its role of proliferation inhibition

作　　者：徐小燕[1] 夏蒲[2] 邢亚楠[3] 张东晗[4] 高野康雄 郑华川[2] 

机构地区：[1]中国医科大学基础医学院病理生理学教研室,辽宁沈阳110001 [2]中国医科大学基础医学院病理学与病理生理学研究所,辽宁沈阳110001 [3]中国医科大学附属第一医院肿瘤外科,辽宁沈阳110001 [4]大连医科大学口腔医学院,辽宁大连116044 [5]日本神奈川县癌中心临床研究所,日本241-0815

出　　处：《细胞与分子免疫学杂志》2011年第5期568-570,共3页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金青年科学基金项目(81001093);辽宁省教育厅科学技术研究项目(L2010649)

摘　　要：目的:研究胃癌患者血清和胃癌细胞培养液上清中分泌型REIC含量及重组REIC对胃癌细胞增殖的影响。方法:ELISA检测血清和细胞上清中的REIC表达;WST-8法检测胃癌细胞的增殖。结果:REIC在胃癌患者中的分泌水平比正常健康人明显降低(P<0.05),并且与肿块大小呈负相关(P<0.05);胃癌细胞系培养液上清中的REIC表达低于正常的胃黏膜细胞(P<0.05);胃癌AGS细胞系转染REIC后,细胞培养液上清中的REIC浓度升高(P<0.05);加入外源性融合的REIC可以抑制胃癌细胞AGS和BGC-823的增殖(P<0.05)。结论:分泌型的REIC在胃癌细胞中表达降低,重组REIC蛋白可抑制胃癌细胞的增殖,REIC蛋白可以作为胃癌早诊、生物学和基因治疗的靶点。AIM： To detect secretory REIC content in serum from the patients with gastric carcinoma and cell culture medium of gastric carcinoma cell lines,and determine the effects of recombinant REIC on proliferation of gastric carcinoma cells.METHODS： ELISA was employed to determine REIC content in serum and supernatant,and WST-8 to detect the proliferation of gastric carcinoma cells.RESULTS： The secretion level of REIC was obviously decreased in serum form gastric carcinoma,compared with healthy individuals（P0.05） and inversely correlated with the tumor size of gastric carcinomas（P0.05）.The REIC content was lower in supernatant from gastric carcinoma cells than that in epithelial cells（P0.05）.After the transfection of REIC-expressing plasmid,the concentration of REIC was increased in supernatant from AGS（P0.05）.The proliferation of AGS and BGC-823 could be inhibited by recombinant REIC（P0.05）.CONCLUSION： REIC expression of secretion type decreased in gastric carcinoma cells,and inhibited the proliferation of gastric carcinoma cells.REIC protein might be employed as a good marker or target for the early diagnosis,biological and gene therapy of gastric carcinoma.

关 键 词：REIC 胃癌 ELISA 血清 

分 类 号：R34[医药卫生—基础医学]