川贝母鳞茎总RNA提取方法的研究  

Study on Extracting Methods of the Total RNA in Bulb of Bulbus Fritillariae Cirrhosae

作　　者：谢紫莹[1] 李玉锋[1] 何洋[2] 刘震东 张波[1] 

机构地区：[1]西华大学生物工程学院,四川成都610039 [2]成都中医药大学,四川成都610075 [3]国嘉集团四川新荷花中药饮片有限公司,四川成都610036

出　　处：《药物生物技术》2011年第3期234-237,共4页Pharmaceutical Biotechnology

基　　金：西华大学食品生物技术省级重点实验室项目

摘　　要：以暗紫贝母(Fritillaria unibracteata Kisao et K.C.Hisa)新鲜鳞茎为试材,选取3种有效提取富含油脂、多糖和酚类植物材料总RNA的方法,比较提取总RNA的效果。结果表明,改良SDS酸酚法能有效去除大部分多糖和DNA,RNA条带清晰、亮度好,28S rRNA的亮度约为18S rRNA的2倍,A260nm/A280nm为1.85,A260nm/A230nm为2.06,得率为73μg/g。提取的总RNA经RT-PCR获得了3-羟基-3-甲基戊二酰辅酶A还原酶(3-hydroxy-3-methylglutaryl-CoA reductase,HMGR)基因的特异性条带,说明改良SDS酸酚法从川贝母中提取的RNA质量好、产率高、完整性强,完全适合于进一步的分子生物学研究。The performance of 3 extracting methods of the total RNA in bulb of Fritillaria unibracteatathat contains large amount of lipin,polysaccharides and phenolic compounds,was compared in this report.The results showed that the modified SDS acid phenol method was capable of efficiently removing polysaccharides,and the brightness of 28S rRNA isolated by this method was two times as much as that of 18S rRNA.A260nm/A280nm,A260nm/A230nm,the RNA productivity was 1.85,2.06 and 73μg/g respectively.The specific RNA bands obtained by RT-PCR explained that the modified SDS acid phenol method could isolate high-quality and highly-complete RNA at a higher productivity and thus it was worth further biological research.

关 键 词：暗紫贝母 油脂 改良SDS酸酚法 多糖 RNA 

分 类 号：S567.231[农业科学—中草药栽培]