新月弯孢霉KA-91对甘草次酸的转化研①究  

Microbial Transformation of Glycyrrhetinic Acid by Curvularia Lunata KA-91

作　　者：和朝军[1,2] 孙华[1] 李鹏举[1] 王敏[1] 

机构地区：[1]天津科技大学生物工程学院工业微生物重点实验室,天津300457 [2]洛阳师范学院生命科学系,河南洛阳471022

出　　处：《药物生物技术》2011年第3期238-241,共4页Pharmaceutical Biotechnology

基　　金：天津市应用基础及前沿技术研究计划重点项目(08JCZDJC15200)

摘　　要：本论文研究了新月弯孢霉KA-91对甘草次酸的微生物转化。该菌株在28℃、180 r/min条件下振荡培养24 h,加入终浓度为0.04%的甘草次酸乙醇溶液。56 h后,停止转化。通过TCL、HPLC、MS1、H NMR和13C NMR分析,转化产物鉴定为7β羟基甘草次酸,转化率为41.1%;该研究丰富了新月弯孢霉作用底物范围,为7β-羟基甘草次酸的合成提供了一条新途径。The biotransformation of glycyrrhetinic acid by Curvularia lunataKA-91 was studied.After the strain was incubated for 24h at 28℃,180r/min,glycyrrhetinic acid was added to 0.04%（m/V）.The conversion reaction was ended after 56h.The purified product was identified as 7β-hydroxyl glycyrrhetinic acid by TCL,HPLC,MS,1H-NMR,and 13↑C-NMR techniques.The yield of 7β-hydroxyl glycyrrhetinic acid was 41.1% under the above biotransformation conditions.This result increased the substrate range of biotransformation by Curvularia lunata KA-91,and provided a novel method to the synthesis of 7β-hydroxyl glycyrrhetinic acid.

关 键 词：甘草次酸 新月弯孢霉 微生物转化 7β-羟基甘草次酸 

分 类 号：Q81[生物学—生物工程] R282.71[医药卫生—中药学]