HPLC测定不同产地白花泡桐中熊果酸和木犀草素的含量  

Quantitative Determination of Ursolic Acid and Luteolin in Paulownia Fortune Produced from Different Places by HPLC

作　　者：李科[1] 吾鲁木汗.那孜尔别克 乔杰[2] 李辉[1] 姚福成 恩特马克.布拉提白 

机构地区：[1]吉首大学植物资源保护与利用湖南省高校重点实验室,湖南吉首416000 [2]国家林业局泡桐研究开发中心,河南郑州450003 [3]湘西宏成制药有限责任公司,湖南吉首416000

出　　处：《药物生物技术》2011年第3期251-255,共5页Pharmaceutical Biotechnology

基　　金：国家自然科学基金项目(30972206);湖南省研究生科研创新项目(CX2009B198);湘西宏成制药公司科技开发项目(XHZ-YP200921)

摘　　要：比较不同产地白花泡桐叶中熊果酸和木犀草素含量。采用Shim-Pack C18色谱柱（4.6 mm×150 mm,5μm）经HPLC法测定含量。测定熊果酸的流动相为甲醇-水-乙酸（85∶15∶0.5）,体积流量为1.0 mL/min,检测波长210 nm;测定木犀草素的流动相为甲醇-水-乙酸（60∶39∶1）,体积流量为1.0 mL/min,检测波长350 nm。结果：熊果酸在1-15μg范围内线性关系良好,回归方程为Y=0.021X＋0.052,r=0.999 6,平均回收率为98.3%;木犀草素在0.01-0.09μg范围内线性关系良好,回归方程为Y=0.001 7X＋0.002 2,r=0.999 1,平均回收率为98.7%。结果显示,宏成制药4号基地白花泡桐叶中熊果酸和木犀草素含量分别为6.57 mg/g和25.5μg/g,显著高于其他地区的样品。本研究结果可为白花泡桐药材质量标准的建立提供实验依据。To compare the contents of ursolic acid and luteolin in the leaves of Paulownia fortuneproduced from different places by HPLC,chromatographic separation of ursolic acid was achieved by using HPLC system consisting of a Shim-Pack C18column（4.6mm×150mm,5μm）,the mobile phase consists of methanol,water and acetic acid（85∶15∶0.5）and detection wavelength is 210nm.On the other hand,chromatographic separation of luteolin was achieved by using HPLC system consisting of a Shim-Pack C18 column（4.6mm×150mm,5μm）,the mobile phase consists of methanol,water and acetic acid（60∶39∶1） and detection wavelength is 350nm.The linearity range of ursolic acid is 1~15μg（r=0.9996） and luteolin is 0.01~ 0.09μg（r=0.9991）.The average recovery rate of ursolic acid is 98.3% and of luleotin is 98.7%.The content of ursolic acid and luteolin in the leaves of Paulownia fortuneat No.4 cultivation place is significantly higher than that of other places,and the content of ursolic acid and luteolin is 6.57mg/g and 25.5μg/g respectively.Results of this study can provide an experimental evidence to establishing the medicine quality standards of Paulownia fortune.

关 键 词：白花泡桐 高效液相色谱法 熊果酸 木犀草素 

分 类 号：R284.1[医药卫生—中药学]