嗜热自养甲烷杆菌的抗逆分子机制  

Molecular Mechanism of Stress Resistance in Methanothermobacter thermoautotrophicus

作　　者：丁霞[1] 彭晓珏[1] 闵航[2] 杨卫军[2] 

机构地区：[1]南昌大学生命科学与食品工程学院,南昌337000 [2]浙江大学生命科学学院,杭州310058

出　　处：《应用与环境生物学报》2011年第3期307-310,共4页Chinese Journal of Applied and Environmental Biology

基　　金：江西省自然科学基金项目(No.2009GQN0091);江西省教育厅基金项目(No.GJJ10048)资助~~

摘　　要：为揭示嗜热厌氧古菌适应极端环境的分子机理,分别在不同温度(50℃,60℃,65℃和70℃)培养嗜热自养甲烷杆菌,利用Real-time RT-PCR研究其二硫键异构酶(mtPDI)mRNA的表达情况.结果显示,mtPDI mRNA在上述情况下都有表达,但当温度偏离菌体最适生长温度(65℃),表达量会提高,在50℃和70℃时达到最大.mtPDI mRNA的表达量在50℃下培养时是最适生长温度(65℃)时的10.3倍,在60℃培养时是最适生长温度(65℃)时的2.7倍,在70℃培养时是最适生长温度(65℃)时的9.3倍.说明mtPDI的表达与温度相关,当偏离最适生长温度时mtPDI mRNA表达上调,以帮助体内蛋白质正确折叠.研究表明mtPDI具有协助嗜热自养甲烷杆菌抵抗外界逆境胁迫的作用.In order to reveal the molecular mechanism of thermophilic and anaerobic archaea,the protein disulfide isomerase（mtPDI） mRNA expression in Methanothermobacter thermoautotrophicus ΔH at different growth temperatures（50 ℃,60 ℃,65 ℃ and 70 ℃） was analysed using real-time PCR.The results showed that the mtPDI mRNA could be expressed at the above temperatures,and the expression increased with temperature increase or reduction from the optimal growth temperature of 65 ℃ and reached the maximum at 50 ℃ and 70 ℃,respectively.The mtPDI mRNA expression at 50 ℃ was 10.3 times higher than that at 65 ℃,the expression at 60 ℃ 2.7 times higher than that at 65 ℃,and the expression at 70 ℃ 9.3 times higher than that at 65 ℃.The results indicated that the level of mtPDI mRNA in M.thermoautotrophicus ΔHT was temperature-dependent.The mtPDI mRNA expression increased to help protein fold correctly in vivo and help the archaea resist stresses.Fig 4,Ref 18

关 键 词：古菌 嗜热自养甲烷杆菌 二硫键异构酶 胁迫 

分 类 号：Q93[生物学—微生物学]