Bt肠毒素基因与plcR基因的相关性及肠毒素对小鼠的急性毒性  

Correlation of Enterotoxin Gene and plcR Gene in Bacillus thuringiensis and Toxicity of Enterotoxin to Mice

作　　者：黄必旺[1] 林晓东[1] 苏芙蓉[1] 关雄[1] 

机构地区：[1]福建农林大学生物农药与化学生物学教育部重点实验室,福州350002

出　　处：《应用与环境生物学报》2011年第3期418-421,共4页Chinese Journal of Applied and Environmental Biology

基　　金：国家"863"计划项目(No.2006AA10A212);科技部农业科技成果转化资金(No.2010GB2C400212);福建省自然科学基金项目(No.2010J01069);福建省高校服务海西建设重点项目(No.0b08b005)资助~~

摘　　要：用PCR方法检测溶血肠毒素BL、肠毒素T和肠毒素S三种肠毒素基因(hblA、becT、entS)及毒素调控基因plcR在30株苏云金芽胞杆菌株(Bt)中的分布.结果表明,含有hblA基因、entS基因、becT基因及plcR基因片段的Bt菌株分别占66.7%、70%、70%和73.3%.其中,含有plcR基因的菌株都至少含有一种肠毒素基因,不含肠毒素基因的菌株也未检测到plcR基因,说明plcR基因与肠毒素基因有着密切的关系.用3个Bt菌株WB9、HD2和HD9(都含有hblA、becT、entS和plcR基因片段,HD2和HD9经RPLA与TECRA肠毒素检测试剂盒检测具有高滴度)对小白鼠进行口服急性毒性试验.结果表明,所有3种供试菌株的发酵上清液对小白鼠行为和健康没有明显影响,对内部器官(心、肝、肺等)也没有产生病理现象.The presence of hemolysin Hb1A gene（hblA）,enterotoxin T gene（bceT）,enterotoxin S gene（entS） and toxin regulatory gene plcR in 30 Bacillus thuringiensis strains was analyzed by PCR.The results showed that hb1A gene sequence could be detected in 20 strains（66.7%）,both bceT and entS gene sequences could be detected in 21 strains（70%）,and plcR gene sequence in 22 strains（73.3%）.The strains that included plcR gene sequence also contained at least one enterotoxin gene sequence,and those that lacked enterotoxin gene sequence also lacked the plcR gene sequence,which indicateed that the relationship of enterotoxin gene and plcR gene was close.The acute oral toxicity of the three Bt strains,WB9,HD2 and HD9（All of them including hblA,bceT,entS and plcR gene sequences,and HD2 and HD9 producing high titres with kits of RPLA and TECRA） to white mice were tested with high doses of ferment suspension.The result showed that there were no abnormal behaviors of mice during the test,and no ill-effects in terms of their conditions and in pathology of their internal organs（heart,liver and lung）.Fig 5,Tab 3,Ref 14

关 键 词：苏云金芽胞杆菌 肠毒素基因 调控基因 急性毒性 

分 类 号：S482.3[农业科学—农药学] S858.91[农业科学—植物保护]