长穗偃麦草EeNAC9基因功能初步研究  

Preliminary Function Research of EeNAC9 Gene from Elytrigia elongata

作　　者：高世庆[1] 王永波[1] 唐益苗[1] 徐蓓[1,2] 马锦绣[1] 陈京瑞[1] 柳珊[1] 张风廷[1] 赵昌平[1] 

机构地区：[1]化京农林科学院杂交小麦工程技术研究中心,北京100097 [2]首都师范大学生命科学学院,北京100048

出　　处：《生物技术通报》2011年第6期47-52,共6页Biotechnology Bulletin

基　　金：国家抗逆转基因重大专项(2008ZX08002-002,2008ZX08002-003,2008ZX08002-004);北京市科技新星项目(2007B056,2008B035);北京农林科学院院青年基金项目;北京市自然基金项目(5102016)

摘　　要：NAC(NAM-ATAF-CUC)转录因子在植物逆境胁迫应答反应中发挥重要作用。通过同源克隆方法从长穗偃麦草中分离到一个NAC转录因子基因EeNAC9(Elytrigia elongate NAM-ATAF-CUC 9),该基因编码274个氨基酸,等电点为9.03;对该蛋白二级结构预测发现主要存在9个螺旋区(helices)和无规则卷曲(coils)。氨基酸疏水性分析表明,该蛋白存在大量的疏水性氨基酸。将该基因构建到PBI121双元植物表达载体上,通过农杆菌介导转化烟草,经PCR分子检测获得T0代35S::EeNAC9转基因烟草阳性植株65株。胁迫培养基上初步功能研究表明,过表达EeNAC9转基因烟草表现出对ABA的敏感性,同时增强了对干旱、高盐的胁迫耐性,从而为小麦抗逆分子育种提供了优良候选基因资源。NAC（NAM-ATAF-CUC） transcription factors play important roles in various stress responses. In this study, one NAC gene named EeNAC9 encoding a NAC protein from Elytrigia elongate was isolated. The putative EeNAC9 protein was composed of 274 amino acids, and an isoelectric point was 9.03. Protein secondary structure showed this protein had 9 helices and other coils. Hydropho- bicity analysis of amino acids displayed that the protein contained a lot of hydrophobic amino acids. Then, EeNAC9 gene was constructed into PBI121 by connection and transferred into tobaccos by Agrobacterium-mediated methods. 65 TO transgenie positive plants were ob- tained by PCR molecular tests. Preliminary function identification showed that overexpression of EeNAC9 gene had an effect on ABA sensitivity and enhanced drought and salt tolerances in transgenic tobaccos. Therefore, EeNAC9 gene could be as an excellent candidate gene resource for wheat anti-adversity molecular breeding.

关 键 词：NAC转录因子 转基因烟草 ABA敏感性 盐胁迫 干旱胁迫 

分 类 号：Q943.2[生物学—植物学] S512.1[农业科学—作物学]