烟草烟酰胺酶基因的克隆及序列分析  

Cloning and Sequence Analysis of Nictinamidase Gene from Flue-cured Tobacco

作　　者：蔡刘体[1] 胡重怡[1] 

机构地区：[1]贵州省烟草科学研究所,贵阳550081

出　　处：《生物技术通报》2011年第6期76-79,共4页Biotechnology Bulletin

基　　金：贵州省烟草专卖局项目(黔烟科2007-03;2009-15)

摘　　要：烟酰胺酶是烟草中烟碱合成途径中的关键酶之一。在植物中,通过烟酰胺酶的催化作用,烟酰胺转化成烟酸,烟酸是合成烟碱的一个重要底物。从烤烟品种南江3号(Nicotiana tobacum cv.Nanjiang-3)均一化全长cDNA文库中克隆获得烟草烟酰胺酶基因cDNA全长序列(命名为T-nic1),GenBank中的登录号为HQ448853,该基因全长为841 bp。利用ORF finder和ProtParam软件分析显示,它包含一个完整的开放读码框,编码一个含有162个氨基酸、等电点为4.94、分子量为18.2 kD的小分子量蛋白。Blast搜索结果及进化分析结果表明,该基因编码的蛋白与毛果芍药、拟南芥中的烟酰胺酶序列具有较高的同源性,其氨基酸序列一致性分别为71%、67%。Nicotinamidase is one of key enzymes in the pathway of nicotina biosynthesis in tobacco. In plants,by the catalyzation of nicotinamidase, the nicotinamide was converted into nicotinate which is one of important substrates in the biosythesis of nicotina. From the full-length cDNA library of the flue-cured tobacco cultivars Nanjiang-3, the fifll length cDNA sequence of tobacco nicotinamidase gene（ named T-nicl ）was cloned and its sequence was analyzed. Analysis by the software ORF finder and ProtParam showed that the T- n/cl included 841 bp,which encoding a protein of 162 amino acids,and its GenBank accession is HQ448853. The results of Blast and phylogenetic analysis showed that T-nicl gene had homology with the nicotinamidase gene of some high plants, T-nicl-encoded protein had high identity with the nicotinamidase protein of Arabidopsis thaliana and Populus trichocarpa, which is 71% ,67%, respectively.

关 键 词：烤烟 烟酰胺酶 基因 克隆 

分 类 号：S572[农业科学—烟草工业] Q943.2[农业科学—作物学]