迟缓爱德华氏菌OppA蛋白的免疫原性及免疫保护分析  

Immunogenicity and immunoprotection of oligopeptide permease A (OppA) of Edwardsiella tarda

作　　者：王妍妍[1,2] 李贵阳[1,2] 李杰[1,2] 肖鹏[1] 莫照兰[1] 

机构地区：[1]中国科学院海洋研究所实验海洋生物学重点实验室,山东青岛266071 [2]中国科学院研究生院,北京100049

出　　处：《海洋科学》2011年第5期19-23,共5页Marine Sciences

基　　金：国家自然科学基金项目(31072245);国家鲆鲽类产业技术体系项目(NYCYTX-50);青岛市科技发展计划项目(08-1-7-2-HY)

摘　　要：迟缓爱德华氏菌(Edwardsiella tarda)是水产养殖动物重要的病原菌,引起鱼类爱德华氏菌病,免疫防治是针对该病的一个有效途径。OppA是迟缓爱德华氏菌的一个寡肽透过酶组分,作者开展OppA的免疫原性及免疫保护性的研究,旨在评价其作为鱼类爱德华氏菌病疫苗候选抗原的可行性。将致病性的迟缓爱德华氏菌LSE40的oppA克隆于表达载体pPROEXHTa,转化入大肠杆菌BL21(DE3),表达重组蛋白6His-OppA,并利用Ni-NTA SefinoseTM kit进行纯化。以纯化的6His-OppA两次注射免疫大菱鲆(Scophthalmus maximus),第34天后检测血清抗体效价为1:1024;以LSE40对免疫的大菱鲆进行人工感染,测得免疫保护率是25.9%。结果表明,OppA可刺激大菱鲆产生免疫应答,并具有免疫保护效果。Edwardsiella tarda is an important pathogen to aquaculture animals, causing fish edwardsiellosis diseases. Vaccination is an efficient protection against this disease in fish. OppA is a component of the oligopeptide permease system of E. tarda. We evaluated the potential of OppA as an antigenic candidate vaccine against ed- wardsiellosis. The oppA gene was cloned into expression vector pPROEXHTa and expressed in E. coli BL21 （DE3）. The expressed 6His-OppA was purified with Ni-NTA SefinoseTM Kit and used as an antigen to immunize turbot （Scophthamus maximus） twice. At day 34 post-vaccination, the serum antibody titer in the immunized fish was 1：1024, and the relative percentage survival （RPS） was 25.9% when the immunized turbot was challenged with pathogenic E. tarda. The present studies indicate that OppA of E. tarda can induce immune response in fish and provide protection against edwardsiellosis.

关 键 词：迟缓爱德华氏菌(Edwardsiella tarda) OppA 原核表达 免疫应答 免疫保护 

分 类 号：Q939.91[生物学—微生物学]