HPLC测定人血浆中头孢噻利的血药浓度  

Determination of Cefoselis in Human Plasma by HPLC

作　　者：华俊彦[1] 朱雅艳[1] 田伟强[1] 赵静[1] 

机构地区：[1]丽水市中心医院药剂科,浙江丽水323000

出　　处：《中国现代应用药学》2011年第6期548-550,共3页Chinese Journal of Modern Applied Pharmacy

基　　金：浙江省医学会基金项目(2009ZYC53)

摘　　要：目的建立高效液相色谱法测定人血浆中头孢噻利血药浓度的方法。方法以甲硝唑为内标,色谱柱为ZORBAXExtend-C18（4.6 mm×250 mm,5μm）,以乙腈-pH 6.5磷酸盐缓冲液（5.5∶94.5）为流动相,流速为1.0 mL.min-1,检测波长为254 nm,进样量为20μL,柱温为25℃。结果头孢噻利的血药浓度在1~200 mg.L-1内线性良好（r=0.999 9）,定量限为1 mg.L-1;平均方法回收率为99.8%（RSD=1.53%）;日内、日间RSD均〈3%。结论本方法简便、灵敏、快速、准确,可用于头孢噻利的临床药动学研究和生物利用度研究。OBJECTIVE To establish an HPLC method for the determination of cefoselis in human plasma. METHODS With metronidazole as the internal standard, the separation of sample was carried out on a ZORBAX Extend C18 column （4.6 mm×250 mm, 5 μm） with the mobile phase consisted of phosphate buffer （pH 6.5）-acetonitrile （94.5∶5.5） at a flow rate of 1.0 mL.min-1. The detection wavelength was 254 nm, injected sample volume was 20 μL, and the column temperature was set at 25 ℃. RESULTS The linear range of cefoselis was 1-200 mg.L-1 （r=0.999 9） with the lower limit of quantization of 1 mg.L-1. The average methodological recovery was 99.8%（RSD=1.53%）. The inter-day RSD and intra-day RSD were all less than 3%. CONCLUSION The method is simple, sensitive, rapid, and accurate, and can be used for clinical pharmacokinetic and bioavailability study of cefoselis.

关 键 词：头孢噻利 高效液相色谱法 血药浓度 

分 类 号：R969.11[医药卫生—药理学]